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Fluorescence In Situ Hybridization (FISH) — Application Guide pp 193–216Cite as

Simultaneous Fluorescence Immunostaining and FISH

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Part of the book series: Springer Protocols Handbooks ((SPH))

A visualization method that combines simultaneous fluorescence immunostaining and FISH represents a powerful means to monitor and visualize DNA–protein interactions in situ. This method has been extensively used in basic chromosomal biology and in medical diagnostics related analysis due to its versatility; it is capable of analyzing the structures, functions, behaviors and abnormalities of chromatin domains, individual chromosomes or specific regions, and entire genomes or various cell populations. In this chapter, detailed protocols that can be applied to study both mitotic and meiotic chromosomes and cells are presented, along with a brief introduction of the methodology and its popular uses in the field of chromosome and genome research. Some technical considerations and possible future directions are also covered.

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Acknowledgments

This work was supported by the fund from the Wayne State University Office of the Vice President for Research and Center for Molecular Medicine and Genetics, as well as the Karmanos Cancer Institute. Additional support came from the Susan Komen Breast Cancer Foundation and the research and development fund from SeeDNA Biotech Inc. We would like to thank SeeDNA Inc. for sharing their experience with us. Special thanks go to Peter Moens and Barbara Spyropoulos for their decades of support. Due to space limitations, we regret that we were unable to cite all of the notable references deserving acknowledgement.

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Authors and Affiliations

  1. Center for Molecular Medicine and Genetics, Wayne State University School of Medicine, Detroit, MI, 48201, USA

    Christine J. Ye, Lesley Lawrenson, Guo Liu, Joshua Stevens & Henry H. Q. Heng

  2. SeeDNA Biotech Inc., Windsor, ON, Canada

    Christine J. Ye

  3. Center for Molecular Medicine and Genetics, Wayne State University School of Medicine, Detroit, MI, 48201, USA

    Henry H. Q. Heng

Authors
  1. Christine J. Ye
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  2. Lesley Lawrenson
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  3. Guo Liu
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  4. Joshua Stevens
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  5. Steve Bremer
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  6. Karen J. Ye
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  7. Henry H. Q. Heng
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Corresponding author

Correspondence to Henry H. Q. Heng .

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Editors and Affiliations

  1. Institut für Humangenetik und Anthropologie, Kollegiengasse 10, Jena, 07743, Germany

    Thomas Liehr

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© 2009 Springer-Verlag Berlin Heidelberg

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Ye, C.J. et al. (2009). Simultaneous Fluorescence Immunostaining and FISH. In: Liehr, T. (eds) Fluorescence In Situ Hybridization (FISH) — Application Guide. Springer Protocols Handbooks. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-70581-9_19

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  • DOI: https://doi.org/10.1007/978-3-540-70581-9_19

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-540-70580-2

  • Online ISBN: 978-3-540-70581-9

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