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Tips and Tricks for mFISH

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Book cover Fluorescence In Situ Hybridization (FISH) — Application Guide

Part of the book series: Springer Protocols Handbooks ((SPH))

Genetic analysis has been vastly improved by the introduction of the fluorescence in situ hybridization (FISH) procedure. Since 1996, multicolor FISH (mFISH) using whole-chromosome painting libraries as probes has evolved into a powerful technology for detecting interchromosomal changes. Nevertheless, mFISH experiments are not always successful, due to the complexity of the hybridization mix, which requires optimization. Here, we describe the preparative and quality control steps that we have established in our labs in order to obtain consistent results.

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Acknowledgments

This work was supported by the Bundesministerium för Verteidigung, FRG (FV: INSAN I 0697V-3800), EU Grant (Mol Cancer Med) CT-2003, the Tumorzentrum Heidelberg/Mannheim, and the German José Carreras Leukemia Research Fund.

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Authors and Affiliations

  1. Deutsches Krebsforschungszentrum (DKFZ), DKFZ/ZMBH Allianz, Abteilung Genetik der Hautkarzinogenese, Im Neuenheimer Feld 280, Heidelberg, 69120, Germany

    Karin M. Greulich-Bode

  2. Institut für Radiobiologie der Bundeswehr in Verbindung mit der, Universität Ulm, Neuherbergstra²e 11, München, 80937, Germany

    Klaus Gilbertz

  3. DSMZ — German Collection of Microorganisms and Cell Cultures, Department of Cell Cultures, Inhoffenstraße 7B, Braunschweig, 38124, Germany

    Roderick A. F. MacLeod

Authors
  1. Karin M. Greulich-Bode
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  2. Maren Kaufmann
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  3. Klaus Gilbertz
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  4. Roderick A. F. MacLeod
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Corresponding author

Correspondence to Karin M. Greulich-Bode .

Editor information

Editors and Affiliations

  1. Institut für Humangenetik und Anthropologie, Kollegiengasse 10, Jena, 07743, Germany

    Thomas Liehr

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© 2009 Springer-Verlag Berlin Heidelberg

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Greulich-Bode, K.M., Kaufmann, M., Gilbertz, K., MacLeod, R.A.F. (2009). Tips and Tricks for mFISH. In: Liehr, T. (eds) Fluorescence In Situ Hybridization (FISH) — Application Guide. Springer Protocols Handbooks. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-70581-9_18

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  • DOI: https://doi.org/10.1007/978-3-540-70581-9_18

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-540-70580-2

  • Online ISBN: 978-3-540-70581-9

  • eBook Packages: Springer Protocols

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