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FISH on Sperms, Spermatocytes and Oocytes

  • Protocol
Book cover Fluorescence In Situ Hybridization (FISH) — Application Guide

Part of the book series: Springer Protocols Handbooks ((SPH))

It is well known that chromosome in situ hybridization allows the unequivocal identification of targeted human somatic chromosomes. Different fluorescence in situ hybridization (FISH) techniques have been developed over the years and, following mitotic studies, meiotic analyses have been performed using these different techniques. The application of FISH protocols to meiotic cells requires the adaptation of standard protocols to the particularities of these cells. Specific sample fixation is usually required, and in some cases samples need to go through particular pretreatments to guarantee successful FISH. The application of FISH to meiotic cytogenetic research has proven to be very useful and has provided significant information about many of the processes that take place during human gametogenesis. The protocols described in this chapter illustrate the processing of different meiotic samples as well as the FISH procedures.

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Acknowledgments

This work was supported by Fondo Investigación Sanitaria (Madrid) (project PI 051834).

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Authors and Affiliations

  1. Unitat de Biologia Cellular i Genética Médica, Facultat de Medicina, Edifici M, Universitat Autönoma de Barcelona, Bellaterra, 08193, Spain

    Maria Oliver-Bonet

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  1. Maria Oliver-Bonet
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Editors and Affiliations

  1. Institut für Humangenetik und Anthropologie, Kollegiengasse 10, Jena, 07743, Germany

    Thomas Liehr

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© 2009 Springer-Verlag Berlin Heidelberg

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Oliver-Bonet, M. (2009). FISH on Sperms, Spermatocytes and Oocytes. In: Liehr, T. (eds) Fluorescence In Situ Hybridization (FISH) — Application Guide. Springer Protocols Handbooks. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-70581-9_16

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  • DOI: https://doi.org/10.1007/978-3-540-70581-9_16

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-540-70580-2

  • Online ISBN: 978-3-540-70581-9

  • eBook Packages: Springer Protocols

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