Interphase cytogenetics using archival tissue samples is a straightforward approach that can be used to obtain cytogenetic information from nuclei of solid tissue samples. It is the only tool available for investigating specific numerical chromosomal aberrations, chromosomal translocations, amplification of oncogenes, or the deletion of tumor suppressor genes in archival tissue samples at the single-cell level, as metaphase spreads obviously cannot be obtained. Here a collection of protocols for archival sectioned and mounted materials as well as extracted nuclei (from formalin-fixed/paraffin-embedded or cryofixed tissues) are presented.
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References
Dhingra K, Sahin A, Supak J, Kim S Y, Hortobagyi G, Hittelman WN (1992) Chromosome in situ hybridization on formalin-fixed mammary tissue using non-isotopic, non-fluorescent probes: technical considerations and biological implications. Breast Cancer Res Treat 23:201–210
Fickelscher I, Starke H, Schulze E, Ernst G, Kosyakova N, Mkrtchyan H, MacDermont K, Sebire N, Liehr T (2007) A further case with a small supernumerary marker chromosome (sSMC) derived from chromosome 1—evidence for high variability in mosaicism in different tissues of sSMC carriers. Prenat Diagn 27:783–785
Fuller CE, Perry A (2002) Fluorescence in situ hybridization (FISH) in diagnostic and investigative neuropathology. Brain Pathol 12:67–86
Hedley DW, Friedlander ML, Taylor IW, Rugg CA, Musgrove EA (1983) Method for analysis of cellular DNA content of paraffin-embedded pathological material using flow cytometry.J Histochem Cytochem 31:1333–1335
Köpf I, Hanson C, Delle U, Verbiené I, Weimarck A (1996) A rapid and simplified technique for analysis of archival formalin-fixed, paraffin-embedded tissue by fluorescence in situ hybridization (FISH). Anticancer Res 16:2533–2536
Liehr T, Stübinger A, Thoma K, Tulusan HA, Gebhart E (1994) Comparative interphase cytoge-netics using FISH on human ovarian carcinomas. Anticancer Res 14:183–188
Liehr T, Grehl H, Rautenstrauss B (1995a) FISH analysis of interphase nuclei extracted from paraffin-embedded tissue. Trends Genet 11:377–378
Liehr T, Grehl H, Rautenstrauss B (1996) A rapid method for FISH analysis on interphase nuclei extracted from cryofixed tissue. Trends Genet 12:505–506
Liehr T, Grehl H, Rautenstrauss B (1997) Molecular diagnosis of PMP22-associated neuropathies using fluorescence in situ hybridization (FISH) on archival peripheral nerve tissue preparations. Acta Neuropathol 94:266–271
Liehr T, Claussen U, Gebhart E (1999) Nucleus extraction from single mounted tissue sections.Genet Anal 15:65–69
Long AA, Komminoth P, Lee E, Wolfe HJ (1993) Comparison of indirect and direct in-situ polymerase chain reaction in cell preparations and tissue sections. Detection of viral DNA, gene rearrangements and chromosomal translocations. Histochemistry 99:151–162
Neubauer S, Liehr T, Tulusan HA, Gebhart E (1994) Interphase cytogenetics by FISH on archival paraffin material and cultured cells of human ovarian tumors. Int J Oncol 4:317–321
Qian JQ, Bostwick DG, Takahashi S, Borell TJ, Brown JA, Lieber MM, Jenkins RB (1996) Comparison of fluorescence in situ hybridization analysis of isolated nuclei and routine histo-logical sections from paraffin-embedded prostatic adenocarcinoma specimens. Am J Pathol 149:1193–1199
Tibiletti MG, Bernasconi B, Dionigi A, Riva C (1999) The applications of FISH in tumor pathology.Adv Clin Path 3:111–118
Wolfe KQ, Herrington CS (1997) Interphase cytogenetics and pathology: A tool for diagnosis and research. J Pathol 181:359–361
Zang KD, Singer H (1967) Chromosomal constitution of meningiomas. Nature 216:84–85
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Supported in part by the DFG (436 ARM 17/11/06, LI 820/15–1).
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Liehr, T., Manvelyan, M. (2009). Characterization of Archived Formalin-Fixed/Paraffin-Embedded or Cryofixed Tissue, Including Nucleus Extraction. In: Liehr, T. (eds) Fluorescence In Situ Hybridization (FISH) — Application Guide. Springer Protocols Handbooks. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-70581-9_15
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