Abstract
Here we describe a protocol of alfalfa (Medicago sativa L.) plastid transformation by which gfp, a gene encoding the green fluorescent protein (GFP), is inserted into plastid genome via particle bombardment and homoplastomic plant is obtained. Plastid engineering is likely to make a significant contribution to the genetic improvement of this crop and the production of vaccines and therapeutic proteins.
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Acknowledgements
This work was supported in part by founding of projects No. 20076021 and No. 20110753 from Science and Technology Department, Jilin Province, China.
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Xing, S., Wei, Z., Wang, Y., Liu, Y., Lin, C. (2014). Integration and Expression of gfp in the Plastid of Medicago sativa L.. In: Maliga, P. (eds) Chloroplast Biotechnology. Methods in Molecular Biology, vol 1132. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-995-6_25
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DOI: https://doi.org/10.1007/978-1-62703-995-6_25
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