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Plastid Transformation in Cabbage (Brassica oleracea L. var. capitata L.) by the Biolistic Process

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Chloroplast Biotechnology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1132))

Abstract

Cabbage (Brassica oleracea L. var. capitata L.) is one of the most important vegetable crops grown worldwide. Scientists are using biotechnology in addition to traditional breeding methods to develop new cabbage varieties with desirable traits. Recent biotechnological advances in chloroplast transformation technology have opened new avenues for crop improvement. In 2007, we developed a stable plastid transformation system for cabbage and reported the successful transformation of the cry1Ab gene into the cabbage chloroplast genome. This chapter describes the methods for cabbage transformation using biolistic procedures. The following sections are included in this protocol: preparation of donor materials, coating gold particles with DNA, biolistic bombardment, as well as the regeneration and selection of transplastomic cabbage plants. The establishment of a plastid transformation system for cabbage offers new possibilities for introducing new agronomic and horticultural traits into Brassica crops.

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References

  1. Dickson MH, Wallace DH (1986) Cabbage breeding. In: Bassett MJ (ed) Breeding vegetable crops. AVI Pub Co, Westport, CT

    Google Scholar 

  2. Food and Agricultural Organization of the United Nations (FAO) (2010) FAOSTAT. http://faostat.fao.org/site/613/default.aspx#ancor. Accessed 1 Nov 2010.

  3. van Poppel G, Verhoeven DT, Verhagen H, Goldbohm RA (1999) Brassica vegetables and cancer prevention. Epidemiology and mechanisms. Adv Exp Med Biol 472:159–168

    Article  PubMed  Google Scholar 

  4. Fang Z, Liu Y, Lou P, Liu G (2005) Current trends in cabbage breeding. J New Seeds 6:75–107

    Article  Google Scholar 

  5. Cardoza V, Stewart CNJ (2004) Brassica biotechnology: progress in cellular and molecular biology. In Vitro Cell Dev Biol Plant 40: 542–551

    Article  CAS  Google Scholar 

  6. Christey MC, Sinclair BK (1992) Regeneration of transgenic kale (Brassica oleracea var. acephala), rape (B. napus) and turnip (B. campestris var. rapifera) plants via Agrobacterium rhizogenes mediated transformation. Plant Sci 87:161–169

    Article  CAS  Google Scholar 

  7. Pius PK, Achar PN (2000) Agrobacterium-mediated transformation and plant regeneration of Brassica oleracea var. capitata. Plant Cell Rep 19:888–892

    Google Scholar 

  8. Tsukazaki H, Kuginuki Y, Aida R, Suzuki T (2002) Agrobacterium-mediated transformation of a doubled haploid line of cabbage. Plant Cell Rep 21:257–262

    Article  CAS  Google Scholar 

  9. Sretenovic-Rajicic T, Ninkovic S, Miljus-Dukic J, Vinterhalter B, Vinterhalter D (2006) Agrobacterium rhizogenes-mediated transformation of Brassica oleracea var. sabauda and B. oleracea var. capitata. Biol Plant 50:525–530

    Article  CAS  Google Scholar 

  10. Cogan N, Harvey E, Robinson H, Lynn J, Pink D, Newbury HJ, Puddephat I (2001) The effects of anther culture and plant genetic background on Agrobacterium rhizogenes-mediated transformation of commercial cultivars and derived doubled-haploid Brassica oleracea. Plant Cell Rep 20:755–762

    Google Scholar 

  11. Metz TD, Dixit R, Earle ED (1995) Agrobacterium tumefaciens-mediated transformation of broccoli (Brassica oleracea var. italica) and cabbage (Brassica oleracea var. capitata). Plant Cell Rep 15:287–292

    CAS  PubMed  Google Scholar 

  12. Jin RG, Liu YB, Tabashnik BE, Borthakur D (2000) Development of transgenic cabbage (Brassica oleracea var. capitata) for insect resistance by Agrobacterium tumefaciens-mediated transformation. In Vitro Cell Dev Biol Plant 36:231–237

    Article  CAS  Google Scholar 

  13. Bhattacharya RC, Viswakarma N, Bhat SR, Kirti PB, Chopra VL (2002) Development of insect-resistant transgenic cabbage plants expressing a synthetic cryIA(b) gene from Bacillus thuringiensis. Curr Sci 83:146–150

    CAS  Google Scholar 

  14. Paul A, Sharma SR, Sresty TVS, Devi S, Bala S, Kumar PS, Saradhi PP, Frutos R, Altosaar I (2005) Transgenic cabbage resistant to diamondback moth. Indian J Biotechnol 4:72–77

    CAS  Google Scholar 

  15. Fang HJ, Li DL, Wang GL, Li YH, Zhu Z, Li XH (1997) An insect resistance transgenic cabbage plant with cowpea trypsin inhibitor (CpTI) gene. Acta Bot Sinica 39:940–945

    CAS  Google Scholar 

  16. Lei JJ, Yang WJ, Yuan SH, Ying FY, Qiong LC (2006) Study on transformation of cysteine proteinase inhibitor gene into cabbage (Brassica oleracea var. capitata L.). Acta Hortic 706:231–238

    CAS  Google Scholar 

  17. Bhattacharya RC, Maheswari M, Dineshkumar V, Kirti PB, Bhat SR (2004) Transformation of Brassica oleracea var. capitata with bacterial betA gene enhances tolerance to salt stress. Sci Horti 100:215–227

    Article  CAS  Google Scholar 

  18. Li X, Peng RH, Fan HQ, Xiong AS, Yao QH, Cheng ZM, Li Y (2005) Vitreoscilla hemoglobin overexpression increases submergence tolerance in cabbage. Plant Cell Rep 23:710–715

    Article  CAS  PubMed  Google Scholar 

  19. Rafat A, Aziz MA, Rashid AA, Abdullah SNA, Kamaladini H, Sirchi MHT, Javadi MB (2009) Optimization of Agrobacterium tumefaciens-mediated transformation and shoot regeneration after co-cultivation of cabbage (Brassica oleracea subsp. capitata) cv. KY Cross with AtHSP101 gene. Sci Horti 121:447–450

    Google Scholar 

  20. Svab Z, Hajdukiewicz P, Maliga P (1990) Stable transformation of plastids in higher plants. Proc Natl Acad Sci U S A 87:8526–8530

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  21. Verma D, Daniell H (2007) Chloroplast vector systems for biotechnology applications. Plant Physiol 145:1129–1143

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  22. Sinagawa-García SR, Tungsuchat-Huang T, Paredes-López O, Maliga P (2009) Next generation synthetic vectors for transformation of the plastid genome of higher plants. Plant Mol Biol 70:487–498

    Article  PubMed  Google Scholar 

  23. Liu CW, Lin CC, Chen JJ, Tseng MJ (2007) Stable chloroplast transformation in cabbage (Brassica oleracea L. var. capitata L.) by particle bombardment. Plant Cell Rep 26:1733–1744

    Article  CAS  PubMed  Google Scholar 

  24. Liu CW, Lin CC, Yiu JC, Chen JJ, Tseng MJ (2008) Expression of a Bacillus thuringiensis toxin (cry1Ab) gene in cabbage (Brassica oleracea L. var. capitata L.) chloroplasts confers high insecticidal efficacy against Plutella xylostella. Theor Appl Genet 117:75–88

    Article  CAS  PubMed  Google Scholar 

  25. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant 15:473–497

    Article  CAS  Google Scholar 

  26. Martin T, Wöhner RV, Hummel S, Willmitzer L, Frommer WB, Gallagher SR (1992) The GUS reporter system as a tool to study plant gene expression. In: Gallagher SR (ed) GUS protocols: using the GUS gene as a reporter of gene expression. Academic, San Diego, pp 23–43

    Google Scholar 

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Acknowledgments

The authors sincerely wish to thank PeyJen Lee for her help with the preparation of the manuscript. This research was supported by funds from the Council of Agriculture, Taiwan, ROC (101AS-9.1.1-FD-Z1 to Menq-Jiau Tseng and Ming-Te Yang).

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Authors and Affiliations

  1. Department of Horticulture, National Chung Hsing University, Taichung, Taiwan, ROC

    Menq-Jiau Tseng & Wan-Ru Chu

  2. Institute of Molecular Biology, National Chung Hsing University, Taichung, Taiwan, ROC

    Ming-Te Yang

  3. Department of Post-Modern Agriculture, Ming Dao University, Chang Hua, Taiwan, ROC

    Cheng-Wei Liu

Authors
  1. Menq-Jiau Tseng
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  2. Ming-Te Yang
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  3. Wan-Ru Chu
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  4. Cheng-Wei Liu
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Editor information

Editors and Affiliations

  1. Rutgers University, Waksman Inst.of Microbiology, Piscataway, New Jersey, USA

    Pal Maliga

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Tseng, MJ., Yang, MT., Chu, WR., Liu, CW. (2014). Plastid Transformation in Cabbage (Brassica oleracea L. var. capitata L.) by the Biolistic Process. In: Maliga, P. (eds) Chloroplast Biotechnology. Methods in Molecular Biology, vol 1132. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-995-6_23

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  • DOI: https://doi.org/10.1007/978-1-62703-995-6_23

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-994-9

  • Online ISBN: 978-1-62703-995-6

  • eBook Packages: Springer Protocols

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