Abstract
Although plastid transformation has attractive advantages and potential applications in plant biotechnology, for long time it has been highly efficient only in tobacco. The lack of efficient selection and regeneration protocols and, for some species, the inefficient recombination using heterologous flanking regions in transformation vectors prevented the extension of the technology to major crops. However, the availability of this technology for species other than tobacco could offer new possibilities in plant breeding, such as resistance management or improvement of nutritional value, with no or limited environmental concerns. Herein we describe an efficient plastid transformation protocol for potato (Solanum tuberosum subsp. tuberosum). By optimizing the tissue culture system and using transformation vectors carrying homologous potato flanking sequences, we obtained up to one transplastomic shoot per bombardment. Such efficiency is comparable to that usually achieved in tobacco. The method described in this chapter can be used to regenerate potato transplastomic plants expressing recombinant proteins in chloroplasts as well as in amyloplasts.
Vladimir T. Valkov and Daniela Gargano contributed equally to this work.
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References
Solomon-Blackburn RM, Barker H (2001) Breeding virus resistant potatoes (Solanum tuberosum): a review of traditional and molecular approaches. Heredity 86:17–35
Milbourne D, Pande B, Byan GJ (2007) Potato. In: Kole C (ed) Pulses, sugar and tuber crops. Springer, Berlin, pp 205–236
Ruf S, Hermann M, Berger IJ, Carrer H, Bock R (2001) Stable genetic transformation of tomato plastids and expression of a foreign protein in fruit. Nat Biotechnol 19:870–875
Dufourmantel N, Pelissier B, Garcon F, Peltier G, Ferullo J-M, Tissot G (2004) Generation of fertile transplastomic soybean. Plant Mol Biol 55:479–489
Kumar S, Dhingra A, Daniell H (2004) Stable transformation of the cotton plastid genome and maternal inheritance of transgenes. Plant Mol Biol 56:203–216
Kanamoto H, Yamashita A, Asao H, Okumura S, Takase H, Hattori M, Yokota A, Tomizawa K-I (2006) Efficient and stable plastid transformation of Lactuca sativa L. cv. Cisco (lettuce). Transgenic Res 15:205–217
Liu CW, Lin CC, Chen JJ, Tseng MJ (2007) Stable chloroplast transformation in cabbage (Brassica oleracea L. var. capitata L.) by particle bombardment. Plant Cell Rep 26:1733–1744
Singh AK, Verma SS, Bansal KC (2010) Plastid transformation in eggplant (Solanum melongena L.). Transgenic Res 19:113–119
Sidorov VA, Kasten D, Pang S-Z, Hajdukiewicz PTJ, Staub JM, Nehra NS (1999) Stable chloroplast transformation in potato: use of green fluorescent protein as a plastid marker. Plant J 19:209–216
Nguyen TT, Nugent G, Cardi T, Dix PJ (2005) Generation of homoplasmic plastid transformants of a commercial cultivar of potato (Solanum tuberosum L.). Plant Sci 168:1495–1500
Gargano D, Vezzi A, Scotti N, Gray JC, Valle G, Grillo S, Cardi T (2005) The complete nucleotide sequence genome of potato (Solanum tuberosum cv Désirée) chloroplast DNA. In 2nd Solanaceae genome workshop 2005, Ischia, Italy, p. 107
Chung H-J, Jung JD, Park H-W, Kim J-H, Cha HW, Min SR, Jeong W-J, Liu JR (2006) The complete chloroplast genome sequences of Solanum tuberosum and comparative analysis with Solanaceae species identified the presence of a 241-bp deletion in cultivated potato chloroplast DNA sequence. Plant Cell Rep 25:1369–1379
Valkov VT, Gargano D, Manna C, Formisano G, Dix PJ, Gray JC, Scotti N, Cardi T (2011) High efficiency plastid transformation in potato and regulation of transgene expression in leaves and tubers by alternative 5′ and 3′ regulatory sequences. Transgenic Res 20:137–151
Yadav NR, Sticklen MB (1995) Direct and efficient plant regeneration from leaf explants of Solanum tuberosum L. cv. Bintje. Plant Cell Rep 14:645–647
Haberlach GT, Cohen B, Reichert N, Baer M, Towill L, Helgeson JP (1985) Isolation, culture and regeneration of protoplasts of potato and several related species. Plant Sci 39:67–74
Acknowledgments
Work in authors’ labs was supported by the European Union (FP5, project “Plastid Factory,” grant no. QLK3-CT-1999-00692; FP6, project “Plastomics,” grant no. LSHG-CT-2003-503238) to TC.
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Valkov, V.T., Gargano, D., Scotti, N., Cardi, T. (2014). Plastid Transformation in Potato: Solanum tuberosum . In: Maliga, P. (eds) Chloroplast Biotechnology. Methods in Molecular Biology, vol 1132. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-995-6_18
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DOI: https://doi.org/10.1007/978-1-62703-995-6_18
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