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Epitope Mapping with Random Phage Display Library

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Monoclonal Antibodies

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1131))

Abstract

Random phage display library is used to map conformational as well as linear epitopes. These libraries are available in varying lengths and with circularization. We provide here a protocol conveying our experience using a commercially available peptide phage display library, which in our hands provides good results.

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References

  1. Jensen-Jarolim E, Leitner A, Kalchhauser H, Zurcher A, Ganglberger E, Bohle B, Scheiner O, Boltz-Nitulescu G, Breiteneder H (1998) Peptide mimotopes displayed by phage inhibit antibody binding to bet v 1, the major birch pollen allergen, and induce specific IgG response in mice. FASEB J 12:1635–1642

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© 2014 Springer Science+Business Media, New York

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Midoro-Horiuti, T., Goldblum, R.M. (2014). Epitope Mapping with Random Phage Display Library. In: Ossipow, V., Fischer, N. (eds) Monoclonal Antibodies. Methods in Molecular Biology, vol 1131. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-992-5_28

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  • DOI: https://doi.org/10.1007/978-1-62703-992-5_28

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-991-8

  • Online ISBN: 978-1-62703-992-5

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