Abstract
Osteoclasts are the only cell type in the body to resorb bone. Osteoclasts play a critical role in physiologic and pathologic bone remodeling. Many genetic mouse models affect the skeleton by regulating osteoclast function directly or indirectly. This protocol describes a procedure for generating osteoclasts from mouse bone marrow cells using macrophage colony stimulating factor and receptor activator of NF-κB ligand.
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Acknowledgements
This work was supported by research grants from the National Institutes of Health PHS awards (AR48697 and AR53586 to L.X., AR 43510 to B.F.B).
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Xing, L., Boyce, B.F. (2014). RANKL-Based Osteoclastogenic Assays from Murine Bone Marrow Cells. In: Hilton, M. (eds) Skeletal Development and Repair. Methods in Molecular Biology, vol 1130. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-989-5_23
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DOI: https://doi.org/10.1007/978-1-62703-989-5_23
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-988-8
Online ISBN: 978-1-62703-989-5
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