Abstract
At least two distinct modalities of cell death, apoptosis and necrosis, can be distinguished based on differences in morphological, biochemical, and molecular changes of dying cells [1, 2]. Cell death is involved both in physiological and pathological conditions of the skeleton: for example, apoptosis is a crucial event during limb development. Therefore, detection of cell death by using a simple stain is a powerful tool to study molecular and cellular mechanisms of skeletal development and repair [3].
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Bortner CD, Oldenburg NBE, Cidlowski JA (1995) The role of DNA fragmentation in apoptosis. Trends Cell Biol 5:21–26
Kerr JFR, Wyllie AH, Currie AR (1972) Apoptosis: a basic biological phenomenon with wide-ranging implications in tissue kinetics. Br J Cancer 26:239–257
Provot S, Zinyk D, Gunes Y et al (2007) Hif-1alpha regulates differentiation of limb bud mesenchyme and joint development. J Cell Biol 177:451–464
Gavrieli Y, Sherman Y, Ben-Sasson SA (1992) Identification of programmed cell death in situ via specific labeling of nuclear DNA fragmentation. J Cell Biol 119:493–501
Loo DT, Rillema JR (1998) Measurement of cell death. In: Mather JP, Barnes D (eds) Methods in cell biology, vol 57. Academic, San Diego, CA, pp 251–264
Loo DT (1998) In situ detection of DNA damage. In: Mather JP, Barnes D (eds) Methods in cell biology, vol 203. Academic, San Diego, CA, pp 21–30
Ansari B, Coates PJ, Greenstein BD et al (1993) In situ end-labeling detects DNA strand breaks in apoptosis and other physiological and pathological states. J Pathol 170:1–8
Schipani E, Ryan HE, Didrickson S et al (2001) Hypoxia in cartilage: HIF-1alpha is essential for chondrocyte growth arrest and survival. Genes Dev 15:2865–2876
Maes C, Araldi E, Haigh K et al (2012) VEGF-independent cell-autonomous functions of HIF-1α regulating oxygen consumption in fetal cartilage are critical for chondrocyte survival. J Bone Miner Res 27:596–609
Pfander D, Kobayashi T, Knight MC et al (2004) Deletion of Vhlh in chondrocytes reduces cell proliferation and increases matrix deposition during growth plate Development. Development 131:2497–2508
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2014 Springer Science+Business Media, LLC
About this protocol
Cite this protocol
Mangiavini, L., Schipani, E. (2014). TUNEL Assay on Skeletal Tissue Sections to Detect Cell Death. In: Hilton, M. (eds) Skeletal Development and Repair. Methods in Molecular Biology, vol 1130. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-989-5_18
Download citation
DOI: https://doi.org/10.1007/978-1-62703-989-5_18
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-988-8
Online ISBN: 978-1-62703-989-5
eBook Packages: Springer Protocols