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Photoactivated Localization Microscopy for Cellular Imaging

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Book cover Super-Resolution Microscopy Techniques in the Neurosciences

Part of the book series: Neuromethods ((NM,volume 86))

Abstract

In a method termed photoactivated localization microscopy (PALM), super-resolution fluorescence imaging can be achieved through the localization of single molecules. This allows the resolution of specific proteins fused to the appropriate fluorescent protein label. Here, we summarize fluorescent proteins suitable for PALM, the technical aspects of multicolor and three-dimensional imaging, and the software packages that are available. Additionally, we highlight several biological applications with an emphasis on neuroscience.

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Authors and Affiliations

  1. Laboratory of Experimental Biophysics (LEB), Ecole Polytechnique Federale de Lausanne (EPFL), Lausanne, Switzerland

    Paulina Achurra, Seamus Holden, Thomas Pengo & Suliana Manley

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  1. Paulina Achurra
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  2. Seamus Holden
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  3. Thomas Pengo
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  4. Suliana Manley
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Editors and Affiliations

  1. Department of Neuro- and Sensory Physiology,University of Göttingen Medical Center, Göttingen, Germany

    Eugenio F. Fornasiero

  2. Department of Neuro- and Sensory Physiology,University of Göttingen Medical Center, Göttingen, Germany

    Silvio O. Rizzoli

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Achurra, P., Holden, S., Pengo, T., Manley, S. (2014). Photoactivated Localization Microscopy for Cellular Imaging. In: Fornasiero, E., Rizzoli, S. (eds) Super-Resolution Microscopy Techniques in the Neurosciences. Neuromethods, vol 86. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-983-3_5

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  • DOI: https://doi.org/10.1007/978-1-62703-983-3_5

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