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Foundations of Sted Microscopy

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Super-Resolution Microscopy Techniques in the Neurosciences

Part of the book series: Neuromethods ((NM,volume 86))

Abstract

This chapter presents the foundations of Sted microscopy with a comparison to its generalization Resolft microscopy and to stochastic imaging methods (Palm, Storm, Fpalm, and alike).

The first section reviews the advantages of optical microscopy, explains the diffraction limit, and shows how the classical resolution limit was finally broken. It also reviews some of the achievements in super-resolution imaging.

The second section explains in depth the principle of Sted microscopy and highlights some special Sted modalities like the use of continuous wave lasers, time-gating, fast imaging with up to 200 frames per second, and combination with fluorescence correlation spectroscopy.

The third section treats some aspects of resolution in the presence of noise, especially in the scope of high-resolution imaging.

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Notes

  1. 1.

    Ultraviolet.

  2. 2.

    Atomic Force Microscopy.

  3. 3.

    Scanning Tunneling Microscopy.

  4. 4.

    Scanning Near Field Optical Microscopy.

  5. 5.

    Tip-Enhanced Raman Spectroscopy.

  6. 6.

    Scanning Ion-Conductance Microscopy.

  7. 7.

    Green Fluorescent Protein.

  8. 8.

    Fluorescein Arsenical Helix Binder.

  9. 9.

    Based on the use of the deoxyribonucleic acid repair protein alkyl guanine DNA alkyl transferase.

  10. 10.

    Based on the use of a modified haloalkane dehalogenase.

  11. 11.

    Image Interference Microscopy.

  12. 12.

    Total Internal Reflection Fluorescence Microscopy.

  13. 13.

    Structured-Illumination Microscopy.

  14. 14.

    Incoherent Interference Illumination Microscopy.

  15. 15.

    The combination of I2M and I3M.

  16. 16.

    A combination of I5M with laterally structured illumination.

  17. 17.

    Standing Wave Fluorescence Microscopy.

  18. 18.

    Harmonic Excitation Light Microscopy.

  19. 19.

    Scanning Patterned Illumination.

  20. 20.

    Scanning Patterned Detection.

  21. 21.

    Image Scanning Microscopy.

  22. 22.

    Stimulated Emission Depletion Microscopy.

  23. 23.

    Ground State Depletion.

  24. 24.

    Reversible Saturable Optical Fluorescence Transitions.

  25. 25.

    Fluorescence Lifetime Imaging.

  26. 26.

    Continuous Wave Lasers.

  27. 27.

    Triplet Relaxation.

  28. 28.

    Dark State Relaxation.

  29. 29.

    Fluorescence Correlation Spectroscopy.

  30. 30.

    Single Plan Illumination Microscopy.

  31. 31.

    Transient Receptor Potential Channel M5.

  32. 32.

    α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor.

  33. 33.

    Ground State Depletion.

  34. 34.

    Saturated Patterned Excitation Microscopy.

  35. 35.

    Saturated Structured-Illumination Microscopy.

  36. 36.

    Photoactivation Localization Microscopy.

  37. 37.

    Stochastic Optical Reconstruction Microscopy.

  38. 38.

    Fluorescence Photoactivation Localization Microscopy.

  39. 39.

    Photoactivation Microscopy with Independently Running Acquisition.

  40. 40.

    Single-Molecule Active-Control Microscopy.

  41. 41.

    Spectral Precision Distance Microscopy/Spectral Position Determination Microscopy.

  42. 42.

    Interferometric Photoactivation Localization Microscopy.

  43. 43.

    Double Helix Photoactivation Localization Microscopy.

  44. 44.

    Direct Stochastic Optical Reconstruction Microscopy.

  45. 45.

    Ground State Depletion with Individual Molecule Return.

  46. 46.

    Single-Molecule Switching Microscopy/Single-Marker Switching Microscopy.

  47. 47.

    Full Width at Half Maximum.

  48. 48.

    Point Spread Function, describing the image of a point object.

  49. 49.

    Continuous Wave Excitation.

  50. 50.

    Gated sted microscopy.

  51. 51.

    Triplet Relaxation.

  52. 52.

    Dark State Relaxation.

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Authors and Affiliations

  1. Wavefront Engineering Microscopy Group, Neurophotonics Laboratory, University Paris Descartes, Sorbonne Paris City, Paris, France

    Marcel A. Lauterbach

  2. MRC Human Immunology Unit, Weatherall Institute of Molecular Medicine, University of Oxford, Oxford, UK

    Christian Eggeling

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  1. Marcel A. Lauterbach
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  2. Christian Eggeling
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  1. Department of Neuro- and Sensory Physiology,University of Göttingen Medical Center, Göttingen, Germany

    Eugenio F. Fornasiero

  2. Department of Neuro- and Sensory Physiology,University of Göttingen Medical Center, Göttingen, Germany

    Silvio O. Rizzoli

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Lauterbach, M.A., Eggeling, C. (2014). Foundations of Sted Microscopy. In: Fornasiero, E., Rizzoli, S. (eds) Super-Resolution Microscopy Techniques in the Neurosciences. Neuromethods, vol 86. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-983-3_3

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