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Alternative Pre-mRNA Splicing

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Spliceosomal Pre-mRNA Splicing

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1126))

Abstract

Alternative pre-mRNA splicing is an integral part of gene regulation in eukaryotes. Here we provide a basic overview of the various types of alternative splicing, as well as the functional role, highlighting how alternative splicing varies across phylogeny. Regulated alternative splicing can affect protein function and ultimately impact biological outcomes. We examine the possibility that portions of alternatively spliced transcripts are the result of stochastic processes rather than regulated. We discuss the implications of misregulated alternative splicing and explore of the role of alternative splicing in human disease.

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Acknowledgment

Research in the Berglund laboratory is supported by NIH (AR059833) and the Myotonic Dystrophy Foundation.

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Authors and Affiliations

  1. Department of Chemistry and Institute of Molecular Biology, University of Oregon, Eugene, OR, USA

    Stacey D. Wagner & J. Andrew Berglund

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  1. Stacey D. Wagner
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  2. J. Andrew Berglund
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Editors and Affiliations

  1. University of California, Irvine, California, USA

    Klemens J. Hertel

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Wagner, S.D., Berglund, J.A. (2014). Alternative Pre-mRNA Splicing. In: Hertel, K. (eds) Spliceosomal Pre-mRNA Splicing. Methods in Molecular Biology, vol 1126. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-980-2_4

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  • DOI: https://doi.org/10.1007/978-1-62703-980-2_4

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-979-6

  • Online ISBN: 978-1-62703-980-2

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