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Protein Downstream Processing pp 89–100Cite as

Aqueous Two-Phase System Strategies for the Recovery of Proteins from Plants

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1129))

Abstract

The increasing interest of the biopharmaceutical industry to exploit plants as a commercially viable production system is demanding the development of new strategies to maximize product recovery. Aqueous two-phase systems (ATPSs) are a primary recovery technique that has shown great potential for the efficient extraction and purification of biological products. The evaluation of different system parameters upon the partitioning behavior can provide the conditions that favor the concentration of contaminants and the desired target protein in opposite phases. The protocols described provide the basic strategy to explore the use of ATPS for the isolation and partial purification of native and recombinant proteins expressed in plants.

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Acknowledgments

The authors wish to acknowledge the financial support of Tecnológico de Monterrey Bioprocesses Research Chair (Grant CAT161).

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Authors and Affiliations

  1. Centro de Biotecnología-FEMSA, Tecnológico de Monterrey, Monterrey, NL, Mexico

    Oscar Aguilar

  2. Centro de Biotecnología-FEMSA, Tecnológico de Monterrey, Campus Monterrey, Ave. Eugenio Garza Sada 2501 Sur, Monterrey, NL, 64849, Mexico

    Marco Rito-Palomares

Authors
  1. Oscar Aguilar
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  2. Marco Rito-Palomares
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Corresponding author

Correspondence to Marco Rito-Palomares .

Editor information

Editors and Affiliations

  1. Laboratory of Enzyme Technology, Agricultural University of Athens Department of Agr. Biotechnology, Athens, Greece

    Nikolaos E. Labrou

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Aguilar, O., Rito-Palomares, M. (2014). Aqueous Two-Phase System Strategies for the Recovery of Proteins from Plants. In: Labrou, N. (eds) Protein Downstream Processing. Methods in Molecular Biology, vol 1129. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-977-2_9

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  • DOI: https://doi.org/10.1007/978-1-62703-977-2_9

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-976-5

  • Online ISBN: 978-1-62703-977-2

  • eBook Packages: Springer Protocols

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