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Deadenylation and Its Regulation in Eukaryotic Cells

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Polyadenylation

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1125))

Abstract

Messenger RNA deadenylation is a process that allows rapid regulation of gene expression in response to different cellular conditions. The change of the mRNA poly(A) tail length by the activation of deadenylation might regulate gene expression by affecting mRNA stability, mRNA transport, or translation initiation. Activation of deadenylation processes are highly regulated and associated with different cellular conditions such as cancer, development, mRNA surveillance, DNA damage response, and cell differentiation. In the last few years, new technologies for studying deadenylation have been developed. Here we overview concepts related to deadenylation and its regulation in eukaryotic cells. We also describe some of the most commonly used protocols to study deadenylation in eukaryotic cells.

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Authors and Affiliations

  1. Department of Chemistry, Hunter College and Graduate Center, City University of New York, 10065, New York, NY, USA

    Xiaokan Zhang, Frida E. Kleiman & Emral Devany

Authors
  1. Xiaokan Zhang
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  2. Frida E. Kleiman
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  3. Emral Devany
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Corresponding author

Correspondence to Frida E. Kleiman .

Editor information

Editors and Affiliations

  1. MRC Mitochondrial Biology Unit, Mitochondrial Genetics Group, Cambridge, United Kingdom

    Joanna Rorbach

  2. Mitochondrial Research Group, Newcastle University The Medical School, Newcastle upon Tyne, United Kingdom

    Agnieszka J. Bobrowicz

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Zhang, X., Kleiman, F.E., Devany, E. (2014). Deadenylation and Its Regulation in Eukaryotic Cells. In: Rorbach, J., Bobrowicz, A. (eds) Polyadenylation. Methods in Molecular Biology, vol 1125. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-971-0_23

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  • DOI: https://doi.org/10.1007/978-1-62703-971-0_23

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-970-3

  • Online ISBN: 978-1-62703-971-0

  • eBook Packages: Springer Protocols

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