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Homing Endonucleases pp 223–243Cite as

Bioinformatics Identification of Coevolving Residues

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1123))

Abstract

Positions in a protein are thought to coevolve to maintain important structural and functional interactions over evolutionary time. The detection of putative coevolving positions can provide important new insights into a protein family in the same way that knowledge is gained by recognizing evolutionarily conserved characters and characteristics. Putatively coevolving positions can be detected with statistical methods that identify covarying positions. However, positions in protein alignments can covary for many other reasons than coevolution; thus, it is crucial to create high-quality multiple sequence alignments for coevolution inference. Furthermore, it is important to understand common signs and sources of error. When confounding factors are accounted for, coevolution is a rich resource for protein engineering information.

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Authors and Affiliations

  1. Department of Biochemistry, University Of Western Ontario, London, ON, Canada

    Russell J. Dickson & Gregory B. Gloor

Authors
  1. Russell J. Dickson
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  2. Gregory B. Gloor
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Editors and Affiliations

  1. University of Western Ontario, London, Ontario, Canada

    David R. Edgell

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© 2014 Springer Science+Business Media New York

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Dickson, R.J., Gloor, G.B. (2014). Bioinformatics Identification of Coevolving Residues. In: Edgell, D. (eds) Homing Endonucleases. Methods in Molecular Biology, vol 1123. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-968-0_15

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  • DOI: https://doi.org/10.1007/978-1-62703-968-0_15

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-967-3

  • Online ISBN: 978-1-62703-968-0

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