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Neutrophil Methods and Protocols pp 517–535Cite as

Diagnostic Assays for Chronic Granulomatous Disease and Other Neutrophil Disorders

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1124))

Abstract

Inasmuch as neutrophils are the primary cellular defense against bacterial and fungal infections, disorders that affect these white cells typically predispose individuals to severe and recurrent infections. Therefore, diagnosis of such disorders is an important first step in directing long-term treatment/care for the patient. Herein, we describe methods to identify chronic granulomatous disease, leukocyte adhesion deficiency, and neutropenia. The assays are relatively simple to perform and cost effective and can be performed with equipment available in most laboratories.

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Authors and Affiliations

  1. Laboratory of Clinical Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA

    Houda Zghal Elloumi & Steven M. Holland

Authors
  1. Houda Zghal Elloumi
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  2. Steven M. Holland
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Editors and Affiliations

  1. Montana State University Dept. Veterinary Molecular Biology, Bozeman, Montana, USA

    Mark T. Quinn

  2. Lab. Human Bacterial Pathogenesis, National Institutes of Health Nat. Inst. Allergy & Infectious Diseases, Hamilton, Montana, USA

    Frank R. DeLeo

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Elloumi, H.Z., Holland, S.M. (2014). Diagnostic Assays for Chronic Granulomatous Disease and Other Neutrophil Disorders. In: Quinn, M., DeLeo, F. (eds) Neutrophil Methods and Protocols. Methods in Molecular Biology, vol 1124. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-845-4_31

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  • DOI: https://doi.org/10.1007/978-1-62703-845-4_31

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-844-7

  • Online ISBN: 978-1-62703-845-4

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