Abstract
This chapter describes two methods for measuring the bactericidal activity of neutrophils. These are a new simple fluorescence-based assay, which quantifies bactericidal activity by measuring changes in bacterial fluorescence associated with a loss of membrane potential over time, and a more traditional colony counting protocol. Two variations of these techniques are presented: a “one-step” protocol providing a composite measure of phagocytosis and killing, and a “two-step” protocol that allows calculation of separate rate constants for both of these processes.
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Acknowledgements
This work was supported by the Health Research Council of New Zealand.
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Parker, H.A., Magon, N.J., Green, J.N., Hampton, M.B., Winterbourn, C.C. (2014). Analysis of Neutrophil Bactericidal Activity. In: Quinn, M., DeLeo, F. (eds) Neutrophil Methods and Protocols. Methods in Molecular Biology, vol 1124. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-845-4_19
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DOI: https://doi.org/10.1007/978-1-62703-845-4_19
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Publisher Name: Humana Press, Totowa, NJ
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Online ISBN: 978-1-62703-845-4
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