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Generation of Functionally Mature Neutrophils from Induced Pluripotent Stem Cells

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Neutrophil Methods and Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1124))

Abstract

Induced pluripotent stem cells (iPSCs) are pluripotent stem cells established from somatic cells. The capability of iPSCs to differentiate into any mature cell lineage under the appropriate conditions allows for modeling of cell processes as well as disease states. Here, we describe an in vitro method for generating functional mature neutrophils from human iPSCs. We also describe assays for testing these differentiated cells for neutrophil characteristics and functions by morphology, cell surface markers, production of reactive oxygen species, microbial killing, and mobilization of neutrophils to an inflammatory site in an in vivo immunodeficient mouse infusion model.

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Acknowledgements

All of the authors were supported by the intramural research program of the National Institute of Allergy and Infectious Diseases. This work was supported in part by an intramural award to HLM from the NIH Center for Regenerative Medicine.

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Authors and Affiliations

  1. Laboratory of Host Defenses, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA

    Colin L. Sweeney, Randall K. Merling, Uimook Choi, Hongmei Wang & Harry L. Malech

  2. Neutrophil Monitoring Lab, Applied/Developmental Research Directorate, Frederick National Laboratory on Cancer Research, Frederick, MD, USA

    Debra Long Priel & Douglas B. Kuhns

Authors
  1. Colin L. Sweeney
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  2. Randall K. Merling
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  3. Uimook Choi
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  4. Debra Long Priel
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  5. Douglas B. Kuhns
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  6. Hongmei Wang
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  7. Harry L. Malech
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Editor information

Editors and Affiliations

  1. Montana State University Dept. Veterinary Molecular Biology, Bozeman, Montana, USA

    Mark T. Quinn

  2. Lab. Human Bacterial Pathogenesis, National Institutes of Health Nat. Inst. Allergy & Infectious Diseases, Hamilton, Montana, USA

    Frank R. DeLeo

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Sweeney, C.L. et al. (2014). Generation of Functionally Mature Neutrophils from Induced Pluripotent Stem Cells. In: Quinn, M., DeLeo, F. (eds) Neutrophil Methods and Protocols. Methods in Molecular Biology, vol 1124. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-845-4_12

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  • DOI: https://doi.org/10.1007/978-1-62703-845-4_12

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-844-7

  • Online ISBN: 978-1-62703-845-4

  • eBook Packages: Springer Protocols

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