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Techniques for Characterizing Cytomegalovirus-Encoded miRNAs

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Human Cytomegaloviruses

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1119))

Abstract

microRNAs (miRNAs) are small noncoding RNAs that regulate gene expression at the posttranscriptional level, by binding to sites within the 3′ untranslated regions of messenger RNA (mRNA) transcripts. The discovery of this completely new mechanism of gene regulation necessitated the development of a variety of techniques to further characterize miRNAs, their expression, and function. In this chapter, we will discuss techniques currently used in the miRNA field to express, detect, and inhibit miRNAs as well as methods used to identify their targets.

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Author information

Authors and Affiliations

  1. Vaccine & Gene Therapy Institute, Oregon Health and Sciences University, Beaverton, OR, USA

    Lauren M. Hook, Igor Landais & Meaghan H. Hancock

  2. Vaccine and Gene Therapy Institute, Oregon Health and Sciences University, 505 NW 185th Avenue, Beaverton, OR, 97006, USA

    Jay A. Nelson

Authors
  1. Lauren M. Hook
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  2. Igor Landais
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  3. Meaghan H. Hancock
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  4. Jay A. Nelson
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Corresponding author

Correspondence to Jay A. Nelson .

Editor information

Editors and Affiliations

  1. Department of Microbiology & Immunology, Louisiana State University Health Scienc, Shreveport, Louisiana, USA

    Andrew D. Yurochko

  2. Department of Molecular Genetics, University of Cincinnati College of Medi, Cincinnati, Ohio, USA

    William E. Miller

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Hook, L.M., Landais, I., Hancock, M.H., Nelson, J.A. (2014). Techniques for Characterizing Cytomegalovirus-Encoded miRNAs. In: Yurochko, A., Miller, W. (eds) Human Cytomegaloviruses. Methods in Molecular Biology, vol 1119. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-788-4_14

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  • DOI: https://doi.org/10.1007/978-1-62703-788-4_14

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-787-7

  • Online ISBN: 978-1-62703-788-4

  • eBook Packages: Springer Protocols

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