Methods for Studying the Function of Cytomegalovirus GPCRs

  • Christine M. O’Connor
  • William E. MillerEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1119)


All of the cytomegaloviruses discovered to date encode two or more genes with significant homology to G-protein coupled receptors (GPCRs). The functions of these cytomegalovirus GPCRs are just beginning to be elucidated; however, it is clear that they exhibit numerous interesting activities in both in vitro and in vivo systems. In this chapter, we review the various methodologies that can be used to examine biochemical aspects of viral GPCR signaling in vitro as well as examine the biological activity of these viral GPCRs in vitro and in vivo in virus infected cells using recombinant cytomegaloviruses.

Key words

G-protein coupled receptors Human cytomegalovirus Murine cytomegalovirus Virus genetics Signal transduction Virological methods 


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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  1. 1.Section of Virology, Department of Molecular Genetics, Lerner Research InstituteThe Cleveland ClinicClevelandUSA
  2. 2.Department of Molecular Genetics, Biochemistry, and MicrobiologyUniversity of Cincinnati College of MedicineCincinnatiUSA

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