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Bioinformatics Analysis and Optimization of Cell-Free Protein Synthesis

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Cell-Free Protein Synthesis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1118))

Abstract

Cell-free protein synthesis offers substantial advantages over cell-based expression, allowing direct access to the protein synthetic reaction and meticulous control over the reaction conditions. Recently, we identified a number of statistically significant correlations between calculated and predicted properties of amino acid sequences and their amenability to heterologous cell-free expression. These correlations can be of practical use for predicting expression success and optimizing cell-free protein synthesis. In this chapter, we describe our approach and demonstrate how computational and predictive bioinformatics can be used to analyze and optimize cell-free protein expression.

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Author information

Authors and Affiliations

  1. RIKEN Systems and Structural Biology Center, Tsurumi, Yokohama, Japan

    Alexander A. Tokmakov & Mikako Shirouzu

  2. Graduate School of Science, Kobe University, Nada, Japan

    Alexander A. Tokmakov & Yasuo Fukami

  3. Research Center for Environmental Genomics, Kobe University, Nada, Japan

    Alexander A. Tokmakov & Yasuo Fukami

  4. RIKEN Systems and Structural Biology Center, Yokohama, Japan

    Atsushi Kurotani & Shigeyuki Yokoyama

  5. Plant Science Center, Yokohama, Japan

    Atsushi Kurotani

  6. Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, Tokyo, Japan

    Atsushi Kurotani

  7. Department of Biophysics and Biochemistry, Graduate School of Science, The University of Tokyo, Bunkyo-ku, Tokyo, Japan

    Shigeyuki Yokoyama

Authors
  1. Alexander A. Tokmakov
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  2. Atsushi Kurotani
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  3. Mikako Shirouzu
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  4. Yasuo Fukami
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  5. Shigeyuki Yokoyama
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Editor information

Editors and Affiliations

  1. Institute for Molecular Bioscience (IMB), The University of Queensland, St. Lucia, Australia

    Kirill Alexandrov

  2. Institute for Molecular Bioscience (IMB), The University of Queensland, St. Lucia, Australia

    Wayne A. Johnston

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Tokmakov, A.A., Kurotani, A., Shirouzu, M., Fukami, Y., Yokoyama, S. (2014). Bioinformatics Analysis and Optimization of Cell-Free Protein Synthesis. In: Alexandrov, K., Johnston, W. (eds) Cell-Free Protein Synthesis. Methods in Molecular Biology, vol 1118. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-782-2_2

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  • DOI: https://doi.org/10.1007/978-1-62703-782-2_2

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-781-5

  • Online ISBN: 978-1-62703-782-2

  • eBook Packages: Springer Protocols

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