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Cell-Free Protein Synthesis in Microfluidic 96-Well Plates

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Cell-Free Protein Synthesis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1118))

Abstract

Cell-free protein synthesis (CFPS) enables rapid protein expression for the structural and functional characterization of proteins. Implementation of CFPS in a microfluidic platform has additional benefits such as reduced reaction volumes and simultaneous expression of multiple proteins. Here, we describe a microfluidic device that is composed of 96 continuous-exchange cell-free protein expression units and produces a protein synthesis yield up to 87 times higher than a conventional batch system.

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Acknowledgements

This work was supported in part by Defense Advanced Research Projects Agency (DARPA), National Science Foundation (OISE-0968313), and the University of Florida via UF Opportunity Fund. Assistance from Dr. Shouguang Jin and help in device fabrication from Daniel Olivero are greatly appreciated.

Author information

Authors and Affiliations

  1. Department of Biomedical Engineering, University of Florida, Gainesville, FL, USA

    Kirsten Jackson & Z. Hugh Fan

  2. Biomedical Engineering Department, Jordan University of Science and Technology, Irbid, Jordan

    Ruba Khnouf

  3. Department of Mechanical and Aerospace Engineering, University of Florida, Gainesville, FL, USA

    Z. Hugh Fan

Authors
  1. Kirsten Jackson
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  2. Ruba Khnouf
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  3. Z. Hugh Fan
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Editor information

Editors and Affiliations

  1. Institute for Molecular Bioscience (IMB), The University of Queensland, St. Lucia, Australia

    Kirill Alexandrov

  2. Institute for Molecular Bioscience (IMB), The University of Queensland, St. Lucia, Australia

    Wayne A. Johnston

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Jackson, K., Khnouf, R., Fan, Z.H. (2014). Cell-Free Protein Synthesis in Microfluidic 96-Well Plates. In: Alexandrov, K., Johnston, W. (eds) Cell-Free Protein Synthesis. Methods in Molecular Biology, vol 1118. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-782-2_10

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  • DOI: https://doi.org/10.1007/978-1-62703-782-2_10

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-781-5

  • Online ISBN: 978-1-62703-782-2

  • eBook Packages: Springer Protocols

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