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Plant Epigenetics and Epigenomics pp 165–175Cite as

Detecting Histone Modifications in Plants

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1112))

Abstract

Histone modifications play an essential role in chromatin-associated processes including gene regulation and epigenetic inheritance. It is therefore very important to quantitatively analyze histone modifications at both the single gene and whole genome level. Here, we describe a robust chromatin immunoprecipitation (ChIP) method for Arabidopsis, which could be adapted for other plant species. This method is compatible with multiple downstream applications including qPCR, tilling arrays, and high-throughput sequencing.

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Author information

Authors and Affiliations

  1. Cell & Developmental Biology, John Innes Centre, Imperial College London, London, SW7 2AZ, UK

    Jie Song

  2. Molecular Genetics Group, University of Utrecht, Utrecht, The Netherlands

    Bas Rutjens

  3. Cell & Developmental Biology, John Innes Centre, Norwich, Norfolk, UK

    Caroline Dean

Authors
  1. Jie Song
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  2. Bas Rutjens
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  3. Caroline Dean
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Editor information

Editors and Affiliations

  1. Genetics & Biotechnology Lab,Plant & Agribiosciences Centre (PABC),School of Natural Sciences,National University of Ireland, Galway, Ireland

    Charles Spillane

  2. Genetics & Biotechnology Lab,Plant & Agribiosciences Centre (PABC),School of Natural Sciences,National University of Ireland, Galway, Ireland

    Peter C. McKeown

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Song, J., Rutjens, B., Dean, C. (2014). Detecting Histone Modifications in Plants. In: Spillane, C., McKeown, P. (eds) Plant Epigenetics and Epigenomics. Methods in Molecular Biology, vol 1112. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-773-0_11

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  • DOI: https://doi.org/10.1007/978-1-62703-773-0_11

  • Published:

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-772-3

  • Online ISBN: 978-1-62703-773-0

  • eBook Packages: Springer Protocols

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