Nuclear Ribosomal RNA Genes: ITS Region

  • Pascale Besse
Part of the Methods in Molecular Biology book series (MIMB, volume 1115)


Despite possible drawbacks (intraspecific polymorphisms and possible fungal contamination), sequencing of the ribosomal RNA gene ITS region remains one of the most popular nuclear sequences used for plant taxonomy and phylogeny. A protocol for PCR amplification and sequencing of this region using universal plant primers is provided.

Key words

Ribosomal DNA ITS Sequencing PCR 



Denis Da Silva and Michel Grisoni (UMR PVBMT Cirad-Université de la Réunion) are acknowledged for their help in the ITS amplification protocol design.


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Copyright information

© Springer New York 2014

Authors and Affiliations

  • Pascale Besse
    • 1
  1. 1.UMR C53 PVBMT Université de la Réunion – CiradUniversité de la RéunionIle de la RéunionFrance

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