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Mitochondrial Genome and Plant Taxonomy

  • Jérôme Duminil
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1115)

Abstract

The lability in size, structure, and sequence content of mitochondrial genome (mtDNA) across plant species has sharply limited its use in taxonomic studies. However, due to the new opportunities offered by the availability of complete mtDNA sequence in plant species and the subsequent development of universal primers, the number of mtDNA-based molecular studies has recently increased. Historically, universal primers have enabled to characterize mtDNA polymorphism mainly by the RFLP technique. This methodology has been progressively replaced by Sanger DNA sequencing, which actually provides the full phylogenetic information content of a DNA fragment (single nucleotide, insertion/deletion, and single sequence repeat length polymorphism). This chapter presents a sequencing working protocol to be routinely used in mtDNA-based phylogenetic studies.

Key words

Cytoplasmic DNA Organelle DNA Botany Phylogeny Plants Sequences Genetic diversity Polymorphism 

Notes

Acknowledgements

Research funded by the FNRS (grants FRFC 2.4.576.07.F and MIS F.4.519.10.F). Many thanks to Michela Di Michele and Esra Kaymac for their comments on a previous version of the article.

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Copyright information

© Springer New York 2014

Authors and Affiliations

  • Jérôme Duminil
    • 1
    • 2
  1. 1.Service Evolution Biologique et Ecologie, CP160/12, Faculté des SciencesUniversité Libre de BruxellesBrusselsBelgium
  2. 2.Biodiversity International, Forest Genetic Resources ProgrammeSub-Regional Office for Central AfricaYaoundéCameroon

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