Abstract
Genomic in situ hybridization (GISH) is an invaluable cytogenetic technique which enables the visualization of whole genomes in hybrids and polyploidy taxa. Total genomic DNA from one or two different species/genome is used as a probe, labeled with a fluorochrome and directly detected on mitotic chromosomes from root-tip meristems. In sugarcane we were able to characterize interspecific hybrids of two closely related species as well as intergeneric hybrids of two closely related genera.
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Acknowledgements
This work was financially supported by BSES Ltd and also previously by the Australian Centre for International Agricultural Research and the Co-operative Research Centre for Sugar Industry Innovation through Biotechnology.
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Piperidis, N. (2014). GISH: Resolving Interspecific and Intergeneric Hybrids. In: Besse, P. (eds) Molecular Plant Taxonomy. Methods in Molecular Biology, vol 1115. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-767-9_16
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DOI: https://doi.org/10.1007/978-1-62703-767-9_16
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-766-2
Online ISBN: 978-1-62703-767-9
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