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GISH: Resolving Interspecific and Intergeneric Hybrids

  • Nathalie Piperidis
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1115)

Abstract

Genomic in situ hybridization (GISH) is an invaluable cytogenetic technique which enables the visualization of whole genomes in hybrids and polyploidy taxa. Total genomic DNA from one or two different species/genome is used as a probe, labeled with a fluorochrome and directly detected on mitotic chromosomes from root-tip meristems. In sugarcane we were able to characterize interspecific hybrids of two closely related species as well as intergeneric hybrids of two closely related genera.

Key words

GISH Fluorochrome Interspecific Intergeneric Genome 

Notes

Acknowledgements

This work was financially supported by BSES Ltd and also previously by the Australian Centre for International Agricultural Research and the Co-operative Research Centre for Sugar Industry Innovation through Biotechnology.

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Copyright information

© Springer New York 2014

Authors and Affiliations

  • Nathalie Piperidis
    • 1
  1. 1.SRA LimitedMackayAustralia

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