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Randomly Amplified Polymorphic DNA (RAPD) and Derived Techniques

  • Kantipudi Nirmal Babu
  • Muliyar Krishna Rajesh
  • Kukkumgai Samsudeen
  • Divakaran Minoo
  • Erinjery Jose Suraby
  • Kallayan Anupama
  • Paul Ritto
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1115)

Abstract

Understanding biology and genetics at molecular level has become very important for dissection and manipulation of genome architecture for addressing evolutionary and taxonomic questions. Knowledge of genetic variation and genetic relationship among genotypes is an important consideration for classification, utilization of germplasm resources, and breeding. Molecular markers have contributed significantly in this respect and have been widely used in plant science in a number of ways, including genetic fingerprinting, diagnostics, identification of duplicates and selecting core collections, determination of genetic distances, genome analysis, developing molecular maps, and identification of markers associated with desirable breeding traits. The application of molecular markers largely depends on the type of markers employed, distribution of markers in the genome, type of loci they amplify, level of polymorphism, and reproducibility of products. Among many DNA markers available, random amplified polymorphic DNA (RAPD) is the simplest and cost-effective and can be performed in a moderate laboratory for most of its applications. In addition RAPDs can touch much of the genome and has the advantage that no prior knowledge of the genome under research is necessary. The recent improvements in the RAPD technique like AP-PCR, SCAR, DAF, SRAP, CAPS, RAMPO, and RAHM can complement the shortcomings of RAPDs and have enhanced the utility of this simple technique for specific applications. Simple protocols for these techniques are presented.

Key words

RAPD AP-PCR SCAR DAF SRAP CAPS RAMPO RAHM DNA fingerprinting Genetic diversity Population and evolutionary genetics 

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Copyright information

© Springer New York 2014

Authors and Affiliations

  • Kantipudi Nirmal Babu
    • 1
  • Muliyar Krishna Rajesh
    • 2
  • Kukkumgai Samsudeen
    • 2
  • Divakaran Minoo
    • 3
  • Erinjery Jose Suraby
    • 1
  • Kallayan Anupama
    • 1
  • Paul Ritto
    • 1
  1. 1.Indian Institute of Spices ResearchKozhikodeIndia
  2. 2.Central Plantation Crops Research InstituteKasaragodIndia
  3. 3.Providence Women’s CollegeKozhikodeIndia

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