Abstract
Using engineered nucleases, such as Zinc Finger Nucleases (ZFNs) or Transcription Activator-Like Effector Nucleases (TALENs), to make targeted genomic modifications has become a common technique to create new model organisms and custom cell lines, and has shown great promise for disease treatment. However, these nucleases have the potential for off-target cleavage that could confound interpretation of experimental results and be detrimental for therapeutic use. Here, we describe a method to test for nuclease cleavage at potential off-target sites predicted by bioinformatics models.
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Acknowledgements
This work was supported by the National Institutes of Health as an NIH Nanomedicine Development Center Award (PN2EY018244 to GB). E.J.F. was additionally supported by an NSF Graduate Research Fellowship under Grant No. DGE-1148903.
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Fine, E.J., Cradick, T.J., Bao, G. (2014). Identification of Off-Target Cleavage Sites of Zinc Finger Nucleases and TAL Effector Nucleases Using Predictive Models. In: Storici, F. (eds) Gene Correction. Methods in Molecular Biology, vol 1114. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-761-7_24
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DOI: https://doi.org/10.1007/978-1-62703-761-7_24
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-760-0
Online ISBN: 978-1-62703-761-7
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