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Enzyme Kinetics in Drug Metabolism pp 229–252Cite as

Principles and Experimental Considerations for In Vitro Transporter Interaction Assays

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1113))

Abstract

Drug transporters are now universally acknowledged as important determinants of the absorption, distribution, metabolism and excretion of both endogenous and exogenous compounds. Altered transporter function, whether due to genetic polymorphism, DDIs, disease, or environmental factors such as dietary constituents, can result in changes in drug efficacy and/or toxicity due to changes in circulating or tissue levels of either drugs or endogenous substrates.

Prediction of whether and to what extent the biological fate of a drug is influenced by drug transporters, therefore, requires in vitro test systems that can accurately predict the risk and magnitude of clinical DDIs. While these in vitro assessments appear simple in theory, practitioners recognize that there are multiple factors that can influence experimental outcomes. A better understanding of these variables, including test compound characteristics, test systems, assay formats, and experimental design will enable clear, actionable steps and translatable outcomes that may avoid unnecessary downstream clinical engagement. This chapter will delineate the role of these variables in improving in vitro assay outcomes.

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Authors and Affiliations

  1. Absorption Systems LP, 436 Creamery Way, Suite 600, Exton, PA, USA

    Sid Bhoopathy, Chris Bode, Vatsala Naageshwaran, Erica A. Weiskircher-Hildebrandt & Ismael J. Hidalgo

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  1. Sid Bhoopathy
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  2. Chris Bode
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  3. Vatsala Naageshwaran
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  4. Erica A. Weiskircher-Hildebrandt
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  5. Ismael J. Hidalgo
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Editor information

Editors and Affiliations

  1. Department of Pharmaceutical Sciences, Temple University School of Pharmacy, Philadelphia, Pennsylvania, USA

    Swati Nagar

  2. Novartis Inst. for BioMedical Research, Cambridge, Massachusetts, USA

    Upendra A. Argikar

  3. Drug Metabolism, Boehringer Ingelheim, Ridgefield, Connecticut, USA

    Donald J. Tweedie

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Bhoopathy, S., Bode, C., Naageshwaran, V., Weiskircher-Hildebrandt, E.A., Hidalgo, I.J. (2014). Principles and Experimental Considerations for In Vitro Transporter Interaction Assays. In: Nagar, S., Argikar, U., Tweedie, D. (eds) Enzyme Kinetics in Drug Metabolism. Methods in Molecular Biology, vol 1113. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-758-7_12

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  • DOI: https://doi.org/10.1007/978-1-62703-758-7_12

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  • Publisher Name: Humana Press, Totowa, NJ

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