Abstract
Riboswitches are RNA-based regulatory devices that mediate ligand-dependent control of gene expression. However, there has been limited success in rationally designing riboswitches. Moreover, most previous riboswitches are confined to a particular gene and only perform one-way regulation. Here, we describe a library screening strategy for efficient creation of ON riboswitches of lacI of Escherichia coli. An ON riboswitch of lacI is then integrated with the lac promoter, generating a hybrid device to achieve portable sequential OFF-and-ON gene regulation.
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Jin, Y., Huang, JD. (2014). Portable Two-Way Riboswitches: Design and Engineering. In: Ogawa, A. (eds) Artificial Riboswitches. Methods in Molecular Biology, vol 1111. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-755-6_7
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DOI: https://doi.org/10.1007/978-1-62703-755-6_7
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Publisher Name: Humana Press, Totowa, NJ
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