Abstract
Artificial riboswitches can be generated by functional coupling of an RNA aptamer for synthetic small molecule to an expression platform. RNA aptamers that can bind strongly and selectively to their target are feasible to be used for obtaining more potent artificial riboswitches. In this chapter, we describe tips and notes for in vitro selection of RNA aptamers targeting synthetic small molecules.
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Acknowledgment
We are indebted to Professor M. Uesugi of Kyoto University for helpful suggestions.
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Murata, A., Sato, Si. (2014). In Vitro Selection of RNA Aptamers for a Small-Molecule Dye. In: Ogawa, A. (eds) Artificial Riboswitches. Methods in Molecular Biology, vol 1111. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-755-6_2
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DOI: https://doi.org/10.1007/978-1-62703-755-6_2
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-754-9
Online ISBN: 978-1-62703-755-6
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