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Tobacco Mosaic Virus Assembled High Aspect Ratio Surfaces

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Virus Hybrids as Nanomaterials

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1108))

Abstract

A modified version of the rod-shaped Tobacco Mosaic Virus (TMV1cys) provides a robust template for the self-assembly and fabrication of high-surface-area materials for numerous applications including batteries and sensors. TMV1cys surface fabrication is facilitated by the addition of a single cysteine residue to the virus coat protein that directs the vertical attachment of the virus particle onto substrate surfaces and enables deposition of functional inorganic layers. Here we describe the production and purification of the virus, its assembly onto suitable surfaces, and coating with metallic nickel and cobalt.

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Acknowledgement

This work was supported by Biochemistry Program of the Army Research Office award W911NF1110138.

Author information

Authors and Affiliations

  1. Institute for Bioscience and Biotechnology Research, University of Maryland, College Park, MD, USA

    Adam D. Brown & James N. Culver

  2. Fischell Department of Bioengineering, University of Maryland, College Park, MD, USA

    Adam D. Brown

Authors
  1. Adam D. Brown
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  2. James N. Culver
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Editor information

Editors and Affiliations

  1. Bio/Molecular Science and Engineering, US Naval Research Laboratory, Washington, District of Columbia, USA

    Baochuan Lin

  2. Bio/Molecular Science and Engineering, US Naval Research Laboratory, Washington, District of Columbia, USA

    Banahalli Ratna

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Brown, A.D., Culver, J.N. (2014). Tobacco Mosaic Virus Assembled High Aspect Ratio Surfaces. In: Lin, B., Ratna, B. (eds) Virus Hybrids as Nanomaterials. Methods in Molecular Biology, vol 1108. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-751-8_9

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  • DOI: https://doi.org/10.1007/978-1-62703-751-8_9

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-750-1

  • Online ISBN: 978-1-62703-751-8

  • eBook Packages: Springer Protocols

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