Abstract
Bottom-up self-assembly techniques are a powerful method of building nanoscale structures in an energy efficient and cost effective manner. The use of biological templates, such as proteins, takes advantage of the monodispersity and precision of naturally evolved systems to produce highly organized assemblies of small molecules and nanoparticles. Here we describe a method whereby arginine residues on a viral coat protein (Tobacco Mosaic Virus) are targeted by bis(p-sulfonatophenyl)phenylphosphine (BSPP)-passivated gold nanoparticles with high specificity to create 22 nm rings.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Kovtyukhova N et al (2002) Layer-by-layer self-assembly strategy for template synthesis of nanoscale devices. Mater Sci Eng C 19: 255–262
Schlick TL, Ding Z, Kovacs EW, Francis MB (2005) Dual-surface modification of the tobacco mosaic virus. J Am Chem Soc 127: 3718–3723
Grabarek Z, Gergely J (1990) Zero-length crosslinking procedure with the use of active esters. Anal Biochem 185:131–135
Nam KT, Peelle BR, Lee S-W, Belcher AM (2004) Genetically driven assembly of nanorings based on the M13 virus. Nano Lett 4:23–27
Blum AS et al (2005) An engineered virus as a scaffold for three-dimensional self-assembly on the nanoscale. Small (Weinheim an der Bergstrasse, Germany) 1:702–706
Kegel WK, Van der Schoot P (2006) Physical regulation of the self-assembly of tobacco mosaic virus coat protein. Biophys J 91: 1501–1512
Perrin DD (1972) Dissociation constants of organic bases in aqueous solution [by] D.D. Perrin: Supplement (Butterworths: 1972). http://books.google.ca/books?id=WWj0PgAACAAJ
Russell V (1997) a. & Ward, M. D. Two-dimensional hydrogen-bonded assemblies: the influence of sterics and competitive hydrogen bonding on the structures of guanidinium arenesulfonate networks. J Mater Chem 7:1123–1133
Best MD, Tobey SL, Anslyn EV (2003) Abiotic guanidinium containing receptors for anionic species. Coord Chem Rev 240:3–15
Zahr OK, Blum AS (2012) Solution phase gold nanorings on a viral protein template. Nano Lett 12:629–633
Incardona NL, McKee S, Flanegan JB (1973) Noncovalent interactions in viruses: characterization of their role in the pH and thermally induced conformational changes in bromegrass mosaic virus. Virology 53:204–214
Wang H, Planchart A, Stubbs G (1998) Caspar carboxylates: the structural basis of tobamovirus disassembly. Biophys J 74:633–638
Fraenkel-Conrat H (1957) Degradation of tobacco mosaic virus with acetic acid. Virology 4:1–4
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2014 Springer Science+Business Media, New York
About this protocol
Cite this protocol
Zahr, O.K., Blum, A.S. (2014). Tobacco Mosaic Virus Capsid Protein as Targets for the Self-Assembly of Gold Nanoparticles. In: Lin, B., Ratna, B. (eds) Virus Hybrids as Nanomaterials. Methods in Molecular Biology, vol 1108. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-751-8_8
Download citation
DOI: https://doi.org/10.1007/978-1-62703-751-8_8
Published:
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-750-1
Online ISBN: 978-1-62703-751-8
eBook Packages: Springer Protocols