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Templated Mineralization by Charge-Modified Cowpea Mosaic Virus

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Virus Hybrids as Nanomaterials

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1108))

Abstract

Templated mineralization of virus particles provides routes to narrowly dispersed nanoparticles that are not readily prepared by other means. The templated mineralization of metal or metal oxide on the external surface of wild-type cowpea mosaic virus (CPMV), a plant virus, is facilitated by increasing the external surface negative charge. This is achieved by the chemical modification of surface lysine groups by succinic anhydride. Hence, for example, treatment of charge-modified CPMV succinamate with a 1:2 mixture of iron(II) and iron(III) salts, followed by raising the pH to 10.2, led to the formation of narrowly dispersed, CPMV-templated, magnetite (Fe3O4) nanoparticles.

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Acknowledgement

This work was supported by the Biotechnology and Biological Sciences Research Council, UK (Core Strategic Grant to the John Innes Centre, D.J.E., and JIC DTG, A. A. A. A.).

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Authors and Affiliations

  1. Department of Chemistry, University of Hull, Hull, UK

    Alaa A. A. Aljabali & David J. Evans

Authors
  1. Alaa A. A. Aljabali
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  2. David J. Evans
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Editor information

Editors and Affiliations

  1. Bio/Molecular Science and Engineering, US Naval Research Laboratory, Washington, District of Columbia, USA

    Baochuan Lin

  2. Bio/Molecular Science and Engineering, US Naval Research Laboratory, Washington, District of Columbia, USA

    Banahalli Ratna

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Aljabali, A.A.A., Evans, D.J. (2014). Templated Mineralization by Charge-Modified Cowpea Mosaic Virus. In: Lin, B., Ratna, B. (eds) Virus Hybrids as Nanomaterials. Methods in Molecular Biology, vol 1108. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-751-8_6

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  • DOI: https://doi.org/10.1007/978-1-62703-751-8_6

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-750-1

  • Online ISBN: 978-1-62703-751-8

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