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Virus Hybrids as Nanomaterials pp 211–230Cite as

Molecular Targeted Viral Nanoparticles as Tools for Imaging Cancer

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1108))

Abstract

Viral nanoparticles (VNPs) are a novel class of bionanomaterials that harness the natural biocompatibility of viruses for the development of therapeutics, vaccines, and imaging tools. The plant virus, cowpea mosaic virus (CPMV), has been successfully engineered to create novel cancer-targeted imaging agents by incorporating fluorescent dyes, polyethylene glycol (PEG) polymers, and targeting moieties. Using straightforward conjugation strategies, VNPs with high selectivity for cancer-specific molecular targets can be synthesized for in vivo imaging of tumors. Here we describe the synthesis and purification of CPMV-based VNPs, the functionalization of these VNPs using click chemistry, and their use for imaging xenograft tumors in animal models. VNPs decorated with fluorescent dyes, PEG, and targeting ligands can be synthesized in one day, and imaging studies can be performed over hours, days, or weeks, depending on the application.

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Acknowledgments

This work was supported by Prostate Cancer Canada Grant #2011-742 to JDL, Natural Sciences and Engineering Research Council of Canada (NSERC) grant #326972 to LGL, and NIH/NIBIB grant R00 EB009105 and Mt. Sinai Foundation to NFS. All experiments were performed in accordance with the regulations and guidelines of the Institutional Animal Care and Use Committee at Case Western Reserve University and at the University of Alberta. We thank Desmond Pink for his photography.

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Authors and Affiliations

  1. Translational Prostate Cancer Research Group, University of Alberta, Edmonton, AB, Canada

    Choi-Fong Cho & John D. Lewis

  2. Department of Biomedical Engineering, Case Western Reserve University, Cleveland, OH, USA

    Sourabh Shukla & Nicole F. Steinmetz

  3. Department of Radiology, Case Western Reserve University, Cleveland, OH, USA

    Sourabh Shukla & Nicole F. Steinmetz

  4. Department of Materials Science and Engineering, Case Western Reserve University, Cleveland, OH, USA

    Sourabh Shukla & Nicole F. Steinmetz

  5. Department of Chemistry, The University of Western Ontario, London, ON, Canada

    Emily J. Simpson & Leonard G. Luyt

  6. Department of Oncology, The University of Western Ontario, London, ON, Canada

    Emily J. Simpson & Leonard G. Luyt

  7. Department of Medical Imaging, The University of Western Ontario, London, ON, Canada

    Emily J. Simpson & Leonard G. Luyt

Authors
  1. Choi-Fong Cho
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  2. Sourabh Shukla
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  3. Emily J. Simpson
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  4. Nicole F. Steinmetz
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  5. Leonard G. Luyt
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  6. John D. Lewis
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Editor information

Editors and Affiliations

  1. Bio/Molecular Science and Engineering, US Naval Research Laboratory, Washington, District of Columbia, USA

    Baochuan Lin

  2. Bio/Molecular Science and Engineering, US Naval Research Laboratory, Washington, District of Columbia, USA

    Banahalli Ratna

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Cho, CF., Shukla, S., Simpson, E.J., Steinmetz, N.F., Luyt, L.G., Lewis, J.D. (2014). Molecular Targeted Viral Nanoparticles as Tools for Imaging Cancer. In: Lin, B., Ratna, B. (eds) Virus Hybrids as Nanomaterials. Methods in Molecular Biology, vol 1108. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-751-8_16

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  • DOI: https://doi.org/10.1007/978-1-62703-751-8_16

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-750-1

  • Online ISBN: 978-1-62703-751-8

  • eBook Packages: Springer Protocols

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