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Chemical Modification of the Inner and Outer Surfaces of Tobacco Mosaic Virus (TMV)

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Virus Hybrids as Nanomaterials

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1108))

Abstract

Viral nanoparticles derived from tobacco mosaic virus (TMV) find applications in various fields. We report the purification and chemical modification of TMV which is a hollow rod-shaped plant viral nanoparticle with modifiable interior and exterior surfaces. We describe methods to isolate TMV from its tobacco plant host for spatially controlled interior and exterior chemical modification and to characterize the resulting TMV hybrid materials.

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Acknowledgements

This work was supported by Mt. Sinai Foundation (to NFS), and NIH/NHLBI T32 5T32HL105338-03 training grant (to MAB).

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Authors and Affiliations

  1. Departments of Biomedical Engineering, Case Western Reserve University, Cleveland, OH, USA

    Michael A. Bruckman

  2. Department of Biomedical Engineering, Case Western Reserve University, Cleveland, OH, USA

    Nicole F. Steinmetz

  3. Department of Radiology, Case Western Reserve University, Cleveland, OH, USA

    Nicole F. Steinmetz

  4. Department of Materials Science and Engineering, Case Western Reserve University, Cleveland, OH, USA

    Nicole F. Steinmetz

Authors
  1. Michael A. Bruckman
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  2. Nicole F. Steinmetz
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Editor information

Editors and Affiliations

  1. Bio/Molecular Science and Engineering, US Naval Research Laboratory, Washington, District of Columbia, USA

    Baochuan Lin

  2. Bio/Molecular Science and Engineering, US Naval Research Laboratory, Washington, District of Columbia, USA

    Banahalli Ratna

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© 2014 Springer Science+Business Media, New York

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Bruckman, M.A., Steinmetz, N.F. (2014). Chemical Modification of the Inner and Outer Surfaces of Tobacco Mosaic Virus (TMV). In: Lin, B., Ratna, B. (eds) Virus Hybrids as Nanomaterials. Methods in Molecular Biology, vol 1108. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-751-8_13

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  • DOI: https://doi.org/10.1007/978-1-62703-751-8_13

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-750-1

  • Online ISBN: 978-1-62703-751-8

  • eBook Packages: Springer Protocols

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