Abstract
Viral nanoblock provides specific positioning of recognition moieties and dye molecules which can be used as a signal-generating element and enhance detection sensitivity. The methods described herein use a 30 nm viral nanoblock to couple a variety of proteins and peptides for the incorporation of recognition elements along with a large number of dye molecules (200). The bioconjugation techniques were adapted and optimized over the years to fabricate nanoparticles that exhibit high fluorescence output while maintaining the selectivity of the target receptors. These complexes can be used for the detection of pathogens and toxins in a single step since both receptor and reporter are in the same viral nanoblock. Its stability and nanometer size allows for its utilization in well-established sensing platforms like microarrays.
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Acknowledgements
The author wants to thank Prof. John E. Johnson for CPMV samples and constant collaboration, colleagues and students for their contributions to our VNB projects, and Dr. Gary Vora for his comments on the manuscript. The work was supported by the Office of Naval Research under NRL basic research core funds.
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Soto, C.M. (2014). A Programmable Fluorescent Viral Nanoblock: Sensing Made Easy in a Single Step. In: Lin, B., Ratna, B. (eds) Virus Hybrids as Nanomaterials. Methods in Molecular Biology, vol 1108. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-751-8_12
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DOI: https://doi.org/10.1007/978-1-62703-751-8_12
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