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Strategies for Measurement of Biotransformation Enzyme Gene Expression

  • Marjorie Romkes
  • Shama C. Buch
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1105)

Abstract

The analysis of gene expression is an integral part of any gene function research. A wide variety of techniques have been developed for this purpose, each with its own advantages and limitations. The following chapter seeks to provide an overview of some of the most recent as well as conventional methods to study gene expression. These approaches include Northern blot analysis, ribonuclease protection assay, reverse transcription polymerase chain reaction, expressed tag sequencing, differential display, cDNA arrays, serial analysis of gene expression, and transcriptome sequencing. The current applications of the information derived from gene expression studies require most of the assays to be adaptable for the quantitative analysis of a large number of samples and endpoints within a short period of time coupled with cost-effectiveness. A comparison of some of these features of each analytical approach as well as their advantages and disadvantages has also been provided.

Key words

mRNA expression RT-PCR TaqMan Serial analysis of gene expression cDNA microarrays Northern blot Ribonuclease protection assay Expressed tag sequencing Differential display Pharmacogenomics Biotransformation enzymes 

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Copyright information

© Humana Press 2014

Authors and Affiliations

  1. 1.Division of Hematology/Oncology, Department of MedicineHillman Cancer CenterPittsburghUSA
  2. 2.Center for Clinical PharmacologyUniversity of PittsburghPittsburghUSA

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