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Molecular Toxicology Protocols pp 33–42Cite as

Assessment of Pathological and Physiological Changes in Mouse Lung Through Bronchoalveolar Lavage

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1105))

Abstract

In animals, environmental exposure such as toxic chemicals and microorganisms or pathophysiological conditions in respiratory system could result in inflammatory response in their lungs. Bronchoalveolar lavage (BAL) is a procedure that can be used to collect samples from animal lungs to efficiently evaluate the immune response by examining both the compositions of cells and fluid from lavage. The profile of inflammatory cells in BAL provides a qualitative description of inflammatory response and the secretion in BAL fluid contains proteins of inflammatory mediators and albumin as a quantitative measurement of inflammation and tissue injury in the lungs. A consistent experimental approach on how to lavage mouse lungs and collect samples is important for a reproducible evaluation of pathological and physiological changes in mouse lung especially for the analysis of inflammation.

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Author information

Authors and Affiliations

  1. Department of Environmental and Occupational Health, University of Pittsburgh, 100 Technology Drive, Bridgeside Point, Pittsburgh, PA, 15219, USA

    Yuanpu Peter Di

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  1. Yuanpu Peter Di
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Correspondence to Yuanpu Peter Di .

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Editors and Affiliations

  1. Occupational Health, University of Pittsburgh Dept. Environmental &, Pittsburgh, Pennsylvania, USA

    Phouthone Keohavong

  2. Dept. of Pharmaceutical Sciences, Nova Southeastern University College of Pharmacy, Fort Lauderdale, Florida, USA

    Stephen G. Grant

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© 2014 Humana Press

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Di, Y.P. (2014). Assessment of Pathological and Physiological Changes in Mouse Lung Through Bronchoalveolar Lavage. In: Keohavong, P., Grant, S. (eds) Molecular Toxicology Protocols. Methods in Molecular Biology, vol 1105. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-739-6_3

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  • DOI: https://doi.org/10.1007/978-1-62703-739-6_3

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-738-9

  • Online ISBN: 978-1-62703-739-6

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