Abstract
Pulsed field gel electrophoresis (PFGE) is one of the older methods for the molecular characterization and comparison of microorganisms including bacteria. Nevertheless, PFGE continues to be recognized as the gold standard for molecular typing due to output spanning >90 % of the bacterial genome and standardized protocols and reagents applicable to a wide range of organisms including S. epidermidis.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsSpringer Nature is developing a new tool to find and evaluate Protocols. Learn more
References
Goering RV (2013) Molecular typing techniques: state of the art. In: Tang YW, Stratton CW (eds) Advanced techniques in diagnostic microbiology, 2nd edn. Springer, New York, NY, pp 239–261
Loman NJ, Misra RV, Dallman TJ et al (2012) Performance comparison of benchtop high-throughput sequencing platforms. Nat Biotechnol 30:434–439
Schatz MC, Langmead B, Salzberg SL (2010) Cloud computing and the DNA data race. Nat Biotechnol 28:691–693
Angiuoli SV, White JR, Matalka M et al (2011) Resources and costs for microbial sequence analysis evaluated using virtual machines and cloud computing. PLoS One 6:e26624
Murchan S, Kaufmann ME, Deplano A et al (2003) Harmonization of pulsed-field gel electrophoresis protocols for epidemiological typing of strains of methicillin-resistant Staphylococcus aureus: a single approach developed by consensus in 10 European laboratories and its application for tracing the spread of related strains. J Clin Microbiol 41:1574–1585
Hunter SB, Vauterin P, Lambert-Fair MA et al (2005) Establishment of a universal size standard strain for use with the PulseNet standardized pulsed-field gel electrophoresis protocols: converting the national databases to the new size standard. J Clin Microbiol 43:1045–1050
Tenover FC, Arbeit RD, Goering RV et al (1995) Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis: criteria for bacterial strain typing. J Clin Microbiol 33:2233–2239
Goering RV (2000) The molecular epidemiology of nosocomial infection: past, present, and future. Rev Med Microbiol 11:145–152
Van Belkum A, Tassios PT, Dijkshoorn L et al (2007) Guidelines for the validation and application of typing methods for use in bacterial epidemiology. Clin Microbiol Infect 13(Suppl 3):1–46
Goering RV, Ribot EM, Gerner-Smidt P (2011) Pulsed-field gel electrophoresis: laboratory and epidemiologic considerations for interpretation of data. In: Persing DH, Tenover FC, Tang YW, Nolte FS, Hayden RT et al (eds) Molecular microbiology, 2nd edn. ASM Press, Washington, DC, pp 167–177
Goering RV (2010) Pulsed field gel electrophoresis: a review of application and interpretation in the molecular epidemiology of infectious disease. Infect Genet Evol 10:866–875
Bielaszewska M, Prager R, Zhang W et al (2006) Chromosomal dynamism in progeny of outbreak-related sorbitol-fermenting enterohemorrhagic Escherichia coli O157:NM. Appl Environ Microbiol 72:1900–1909
Goering RV, Tenover FC (1997) Epidemiological interpretation of chromosomal macro-restriction fragment patterns analyzed by pulsed-field gel electrophoresis. J Clin Microbiol 35:2432
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2014 Springer Science+Business Media, LLC
About this protocol
Cite this protocol
Goering, R.V., Fey, P.D. (2014). Pulsed Field Gel Electrophoresis of Staphylococcus epidermidis . In: Fey, P. (eds) Staphylococcus Epidermidis. Methods in Molecular Biology, vol 1106. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-736-5_4
Download citation
DOI: https://doi.org/10.1007/978-1-62703-736-5_4
Published:
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-735-8
Online ISBN: 978-1-62703-736-5
eBook Packages: Springer Protocols