Abstract
Shaking technology in combination with small-scale disposable plastic vessels has become a notable bioprocess optimization tool widely exploited for cells grown in suspension. This chapter focuses on the two most accommodating culture systems: 50 mL centrifugation tubes and 96-deepwell plates. Used by many laboratories for routine passaging of suspension cultures and all types of optimization experiments as flexible culture system, the 50 mL shake tubes are the preferred vessels for manual manipulations, while microtiter plates are the ideal containers when automatic liquid handling systems are available. Both culture systems can offer to the cells a well-mixed environment which is close to the conditions found at larger scale in production bioreactors.
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References
Kloeckner W, Buechs J (2012) Advances in shaking technologies. Trends Biotechnol 30:307–314
Duetz WA (2007) Microtiter plates as mini-bioreactors: miniaturization of fermentation methods. Trends Microbiol 15:469–475
Jordan M, De Jesus MJ, Eigenmann C et al (2005) A versatile disposable culture system for high throughput screening of process parameters and production cell lines. In: Godia F, Fussenegger M (eds) ESACT proceedings. Animal cell technology meets genomics. pp 381–383
Huynh HT, Chan LCL, Tran TTB et al (2012) Improving the robustness of a low-cost insect cell medium for baculovirus biopesticides production, via hydrolysate streamlining using a tube bioreactor-based statistical optimization routine. Biotechnol Prog 28:788–802
DeJesus MJ, Girard P, Bourgeois M et al (2004) TubeSpin satellites: a fast track approach for process development with animal cells using shaking technology. Biochem Eng J 17:217–223
Xie Q, Michel PO, Baldi L et al (2011) TubeSpin bioreactor 50 for the high-density cultivation of Sf-9 insect cells in suspension. Biotechnol Lett 33:897–902
Tissot S, Michel PO, Hacker DL et al (2012) kLa as a predictor for successful probe-independent mammalian cell bioprocesses in orbitally shaken bioreactors. New Biotechnol 29:387–394
Meyer A, Condon RG, Keil G et al (2011) Fluorinert, an oxygen carrier, improves cell culture performance in deep square 96-well plates by facilitating oxygen transfer. Biotechnol Prog 28:171–178
Bareither R, Pollard D (2011) A review of advanced small-scale parallel bioreactor technology for accelerated process development: current state and future need. Biotechnol Prog 27:2–14
Wang Z, Belovich JM (2010) A simple apparatus for measuring cell settling velocity. Biotechnol Prog 26:1361–1366
Barett TA, Wu A, Zhang H et al (2009) Microwell engineering characterization for mammalian cell culture process development. Biotechnol Bioeng 105:260–275
Tissot S, Oberbek A, Reclari M et al (2011) Efficient and reproducible mammalian cell bioprocesses without probes and controllers? New Biotechnol 28:382–390
Xie K, Zhang XW, Huang L et al (2011) On-line monitoring of oxygen in Tubespin, a novel, small-scale disposable bioreactor. Cytotechnology 63:345–350
Funke F, Diederichs S, Kensy F et al (2009) The baffled microtiter plate: increased oxygen transfer and improved online monitoring in small scale fermentations. Biotechnol Bioeng 103:1118–1128
Zagari F, Jordan M, Stettler M et al (2012) Lactate metabolism shift in CHO cells culture: the role of mitochondrial oxidative activity. New Biotechnol 30:238–245
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Jordan, M., Stettler, M. (2014). Tools for High-Throughput Process and Medium Optimization. In: Pörtner, R. (eds) Animal Cell Biotechnology. Methods in Molecular Biology, vol 1104. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-733-4_6
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DOI: https://doi.org/10.1007/978-1-62703-733-4_6
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Publisher Name: Humana Press, Totowa, NJ
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