Abstract
Microbioreactors are increasingly used within animal cell biotechnology to grow mammalian cells for cell line screening and to facilitate process development. Many such devices have been reported in the literature, but only a small number are available commercially. Microbioreactors range in complexity from simple plate-based systems to complex automated parallel bioreactors designed to enable the meaningful scale-down of conventional bioprocesses. The Micro24 MicroReactor system (Pall Life Sciences) fits between these extremes providing 24× 7 mL parallel “bioreactors” with individual monitoring and control of temperature, pH, and dissolved oxygen. Inoculation, sampling, and feed additions are carried out manually in a Biological Safety Cabinet. In this chapter we describe the use of the Micro24 system to carry out screening or process development experiments with CHO cells.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Rohe P, Venkanna D, Kleine B et al (2012) An automated workflow for enhancing microbial bioprocess optimization on a novel microbioreactor platform. Microb Cell Fact 11:144. doi:10.1186/1475-2859-11-144
Kim BJ, Diao J, Shuler ML (2012) Mini-scale bioprocessing systems for highly parallel animal cell cultures. Wiley Online Library. doi:10.1002/btpr.1554
Diao J, Young L, Zhou P, Shuler ML et al (2007) An actively mixed mini-bioreactor for protein production from suspended animal cells. Biotechnol Bioeng 100:72–81
Cui ZF, Xu X, Trainor N et al (2007) Application of multiple parallel perfused microbioreactors and three-dimensional stem cell culture for toxicity testing. Toxicol In Vitro 21:1318–1324
Marques MPC, Fernandes P (2011) Microfluidic devices: useful tools for bioprocess intensification. Molecules 16:8368–8401
Duetz WA (2007) Microtiter plates as mini-bioreactors: miniaturization of fermentation methods. Trends Microbiol 15:469–475
Betts JI, Baganz F (2006) Miniature bioreactors: current practices and future opportunities. Microb Cell Fact 5:21
Schäpper D, Alam MNHZ, Szita N et al (2009) Application of microbioreactors in fermentation process development: a review. Anal Bioanal Chem 395:679–695
Bareither R, Pollard D (2010) A review of advanced small-scale parallel bioreactor technology for accelerated process development: current state and future need. Wiley Online Library. doi:10.1002/btpr.522
Gernaey KV, Baganz F, Franco-Lara E et al (2012) Monitoring and control of microbioreactors: an expert opinion on development needs. Biotechnol J 7:1308–1314
Duetz WA, Rüedi L, Hermqann R et al (2000) Methods for intense aeration, growth, storage and replication of bacterial strains in microtiter plates. Appl Environ Microbiol 66:2641–2646
Betts JPJ, Warr SRC, Finka GB et al (2013) Impact of aeration strategies on fed-batch cell culture kinetics in a single-use 24-well miniature bioreactor. Biochem Eng J (In press)
Chen A, Chitta R, Chang D et al (2009) Twenty-four well plate miniature bioreactor system as a scale-down model for cell culture process development. Biotechnol Bioeng 102:148–160
Warr SRC, Patel J, Ho R, Newell KV (2011) Use of micro bioreactor systems to streamline cell line evaluation and upstream process development for monoclonal antibody production. BMC Proc 5(Suppl 8):14–16
Pall Life Sciences (2012) Micro-24 Microreactor System. New User Training Course. Version 1.1. Pall Group. Supplied with Instrument
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2014 Springer Science+Business Media, LLC
About this protocol
Cite this protocol
Warr, S.R.C. (2014). Microbioreactors and Scale-Down Models: Growth of CHO Cells Using the Pall Micro24 MicroReactor System. In: Pörtner, R. (eds) Animal Cell Biotechnology. Methods in Molecular Biology, vol 1104. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-733-4_11
Download citation
DOI: https://doi.org/10.1007/978-1-62703-733-4_11
Published:
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-732-7
Online ISBN: 978-1-62703-733-4
eBook Packages: Springer Protocols