Abstract
Complement C5 is cleaved by proteolysis in the terminal phase of complement activation generating the pro-inflammatory C5a and membrane attack complex nucleator C5b. Whereas purification of its paralogues C3 and C4 from plasma is relatively straightforward, C5 purification is more complicated due to the lower amounts present and overlaps with the much more abundant C3 during several chromatographic steps. Here we describe our procedure for purifying homogenous, monodisperse, and crystallizable C5.
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Acknowledgement
We thank Lene Kristensen for excellent technical assistance. This work was supported by the Novo-Nordisk Foundation, the Lundbeck Foundation, and the Danish Natural Science Research Council.
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Sottrup-Jensen, L., Andersen, G.R. (2014). Purification of Human Complement Protein C5. In: Gadjeva, M. (eds) The Complement System. Methods in Molecular Biology, vol 1100. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-724-2_7
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DOI: https://doi.org/10.1007/978-1-62703-724-2_7
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Publisher Name: Humana Press, Totowa, NJ
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