Abstract
The complement receptor 2 (Cr2) gene is exclusively expressed in B cells and follicular dendritic cells (FDC) in mice and in humans. CR2 is a receptor for the complement component 3 (C3) cleavage fragments C3d(g) and iC3b. On B cells CR2 acts as the B cell co-receptor in which ligand binding of CR2 effectively lowers the threshold for B cell activation. This protocol describes methods for the functional analysis of calcium signaling enhancement provided by CR2 co-receptor activity.
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Donius, L.R., Weis, J.H. (2014). Quantification of Complement Receptor 2 Calcium Signaling Enhancement Using Flow Cytometry. In: Gadjeva, M. (eds) The Complement System. Methods in Molecular Biology, vol 1100. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-724-2_25
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DOI: https://doi.org/10.1007/978-1-62703-724-2_25
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-723-5
Online ISBN: 978-1-62703-724-2
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