Abstract
Evaluation of total complement function in human serum is an essential component of laboratory diagnostics of the human complement system. During recent years, established hemolytic assays for classical pathway and alternative pathway function, CH50 and AP50 assays, respectively, have been replaced in many diagnostic laboratories by ELISA assays. Next to an improved standardization, this assay platform also allows for functional analysis of the lectin pathway of complement. The present chapter describes the methodology of ELISA assays for assessment of the classical pathway, the alternative pathway, the MBL-dependent lectin pathway, and the Ficolin-3-dependent lectin pathway of complement in clinical laboratory diagnostics.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Skattum L, van Deuren M, van der Poll T et al (2011) Complement deficiency states and associated infections. Mol Immunol 48: 1643–1655
Degn SE, Jensenius JC, Thiel S (2011) Disease-causing mutations in genes of the complement system. Am J Hum Genet 88:689–705
Daha NA, Banda NK, Roos A et al (2011) Complement activation by (auto-)antibodies. Mol Immunol 48:1656–1665
Fredrikson GN, Truedsson L, Sjöholm AG (1993) New procedure for the detection of complement deficiency by ELISA. Analysis of activation pathways and circumvention of rheumatoid factor influence. J Immunol Methods 166:263–270
Petersen SV, Thiel S, Jensen L et al (2001) An assay for the mannan-binding lectin pathway of complement activation. J Immunol Methods 257:107–116
Roos A, Bouwman LH, Munoz J et al (2003) Functional characterization of the lectin pathway of complement in human serum. Mol Immunol 39:655–668
Seelen MA, Roos A, Wieslander J et al (2005) Functional analysis of the classical, alternative, and MBL pathways of the complement system: standardization and validation of a simple ELISA. J Immunol Methods 296:187–198
Munthe-Fog L, Hummelshoj T, Honore C et al (2009) Immunodeficiency associated with FCN3 mutation and ficolin-3 deficiency. N Engl J Med 360:2637–2644
Hein E, Honore C, Skjoedt MO et al (2010) Functional analysis of Ficolin-3 mediated complement activation. PLoS One 5:e15443
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2014 Springer Science+Business Media, New York
About this protocol
Cite this protocol
Roos, A., Wieslander, J. (2014). Evaluation of Complement Function by ELISA. In: Gadjeva, M. (eds) The Complement System. Methods in Molecular Biology, vol 1100. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-724-2_2
Download citation
DOI: https://doi.org/10.1007/978-1-62703-724-2_2
Published:
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-723-5
Online ISBN: 978-1-62703-724-2
eBook Packages: Springer Protocols