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Purification and Functional Analysis of Human Properdin

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The Complement System

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1100))

Abstract

Properdin is a member of the alternative pathway of complement. It is unique in that it is the only known positive regulator of the complement system. Properdin can stabilize and promote complement activation, but in addition is also capable of initiating the alternative pathway, making it a unique protein. This chapter focuses on the methods required for purifying human properdin and testing its functionality.

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Acknowledgments

This work was supported by a grant from the European Union (TranSVIR FP7-PEOPLE-ITN-2008 #238756).

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Authors and Affiliations

  1. Department of Nephrology, Leiden University Medical Centre, Leiden, The Netherlands

    Joseph O’Flynn, Maria C. Faber Krol, Mohamed R. Daha & Cees van Kooten

Authors
  1. Joseph O’Flynn
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  2. Maria C. Faber Krol
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  3. Mohamed R. Daha
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  4. Cees van Kooten
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Editor information

Editors and Affiliations

  1. Division of Infectious Diseases, Brigham and Women’s Hospital, Harvard University Harvard Medical School, Boston, Massachusetts, USA

    Mihaela Gadjeva

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© 2014 Springer Science+Business Media, New York

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O’Flynn, J., Krol, M.C.F., Daha, M.R., van Kooten, C. (2014). Purification and Functional Analysis of Human Properdin. In: Gadjeva, M. (eds) The Complement System. Methods in Molecular Biology, vol 1100. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-724-2_13

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  • DOI: https://doi.org/10.1007/978-1-62703-724-2_13

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-723-5

  • Online ISBN: 978-1-62703-724-2

  • eBook Packages: Springer Protocols

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