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Cell-Based Bioluminescence Screening Assays

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Bioluminescent Imaging

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1098))

Abstract

Drug screening is an essential and widely used technique for drug discovery in various biomedical fields notably in oncology. Here we describe a functional screening assay based on the bioluminescence detection of a secreted luciferase for monitoring cell viability of cancer cells in a high-throughput format. This assay allows the screening of large libraries comprising thousands of compounds and the identification of potential anticancer molecules in a rapid, facile, and cost-effective manner.

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Author information

Authors and Affiliations

  1. Experimental Therapeutics and Molecular Imaging Laboratory, Neuroscience Center, Department of Neurology, Massachusetts General Hospital, Boston, MA, USA

    Romain J. Amante

  2. Program in Neuroscience, Harvard Medical School, Boston, MA, USA

    Romain J. Amante & Christian E. Badr

  3. Experimental Therapeutics and Molecular Imaging Laboratory, Department of Neurology, Neuroscience Center, Massachusetts General Hospital, Boston, MA, USA

    Christian E. Badr

Authors
  1. Romain J. Amante
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  2. Christian E. Badr
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Editor information

Editors and Affiliations

  1. Department of Neurology, Neuroscience Center, Massachusetts General Hospital, Boston, Massachusetts, USA

    Christian E. Badr

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© 2014 Springer Science+Business Media New York

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Amante, R.J., Badr, C.E. (2014). Cell-Based Bioluminescence Screening Assays. In: Badr, C. (eds) Bioluminescent Imaging. Methods in Molecular Biology, vol 1098. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-718-1_15

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  • DOI: https://doi.org/10.1007/978-1-62703-718-1_15

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-717-4

  • Online ISBN: 978-1-62703-718-1

  • eBook Packages: Springer Protocols

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