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Single-Cell Raman Sorting

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Book cover Environmental Microbiology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1096))

Abstract

Single-cell Raman spectroscopy is a noninvasive and label-free technology for biochemical analysis of bacterial cells. A single-cell Raman spectrum functions as a metabolic “fingerprint” of an individual cell, which enables differentiation of cell types, physiological states, nutrient condition, and variable phenotypes. Raman tweezers combines single-cell Raman spectroscopy with optical laser tweezers to allow the identification and isolation of single living cells according to their Raman spectra. After cell sorting subsequent culturing and genomic sequencing has the potential to reveal totally new groups of microbes. Stable isotope probing with Raman tweezers offers a culture-independent toolbox to study genetic functions and physiology of unculturable microorganisms in the ecosystem.

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Acknowledgements

We thank Dr. Simon FitzGerald in HORIBA Scientific Ltd. for his technical input and helpful discussion. We are indebted to the Central Laser Facility at the Rutherford Appleton Laboratory for providing access to the instrument.

Author information

Authors and Affiliations

  1. Kroto Research Institute, Sheffield University, Sheffield, UK

    Mengqiu Li, Dan G. Boardman & Wei E. Huang

  2. Central Laser Facility, Research Complex at Harwell, Science and Technology Facilities Council, Rutherford Appleton Laboratory, Harwell Oxford, Didcot, Oxfordshire, UK

    Andrew Ward

Authors
  1. Mengqiu Li
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  2. Dan G. Boardman
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  3. Andrew Ward
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  4. Wei E. Huang
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Editor information

Editors and Affiliations

  1. Dept. Chemistry & Biomolecular Sciences, Macquarie University, Sydney, New South Wales, Australia

    Ian T. Paulsen

  2. School of Molecular Bioscience, University of Sydney, Camperdown, New South Wales, Australia

    Andrew J. Holmes

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Li, M., Boardman, D.G., Ward, A., Huang, W.E. (2014). Single-Cell Raman Sorting. In: Paulsen, I., Holmes, A. (eds) Environmental Microbiology. Methods in Molecular Biology, vol 1096. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-712-9_12

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  • DOI: https://doi.org/10.1007/978-1-62703-712-9_12

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-711-2

  • Online ISBN: 978-1-62703-712-9

  • eBook Packages: Springer Protocols

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