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Detection of microRNA Maturation Using Unmodified pre-microRNA and Branched Rolling Circle Amplification

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miRNA Maturation

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1095))

Abstract

The ever-increasing number of different miRNAs and their association with a vast number of cellular dysfunctions and diseases have initiated several groups to investigate miRNA maturation, which ultimately leads to down regulation of a target messenger RNA (mRNA) and its downstream product. A rapid, convenient, and reliable assay to detect the Dicer-mediated miRNA-maturation step may facilitate research in this field. Here we describe the in vitro detection of the Dicer-mediated miRNA maturation step using unmodified pre-miRNA and branched rolling circle amplification.

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Acknowledgement

The authors would like to thank the Deutsche Forschungsgemeinschaft (DFG) for ongoing generous support.

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Authors and Affiliations

  1. Institute for Chemistry, Humboldt Universität zu Berlin, Berlin, Germany

    Saskia Neubacher & Christoph Arenz

Authors
  1. Saskia Neubacher
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  2. Christoph Arenz
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Editor information

Editors and Affiliations

  1. Institute for Chemistry, Humboldt Universität zu Berlin, Berlin, Berlin, Germany

    Christoph Arenz

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Neubacher, S., Arenz, C. (2014). Detection of microRNA Maturation Using Unmodified pre-microRNA and Branched Rolling Circle Amplification. In: Arenz, C. (eds) miRNA Maturation. Methods in Molecular Biology, vol 1095. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-703-7_9

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  • DOI: https://doi.org/10.1007/978-1-62703-703-7_9

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-702-0

  • Online ISBN: 978-1-62703-703-7

  • eBook Packages: Springer Protocols

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